Curated Information
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PubMed Id: 11443134 
This page summarizes selected information from the article referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Reusch HP, et al. (2001) Regulation of Raf by Akt controls growth and differentiation in vascular smooth muscle cells. J Biol Chem 276, 33630-7 11443134
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
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S473-p - Akt1 (rat) ▲
Orthologous residues
Akt1 (human): S473‑p, Akt1 (mouse): S473‑p, Akt1 (rat): S473‑p, Akt1 (fruit fly): S586‑p, Akt1 (cow): S473‑p
Characterization
 Methods used to characterize site in vivo:  phospho-antibody
 Relevant cell lines - cell types - tissues:  vascular smooth muscle cell ('muscle, smooth')
 Cellular systems studied:  cell lines
 Species studied:  rat
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
PDGF increase
LY294002 PDGF inhibit treatment-induced increase
thrombin increase
LY294002 thrombin inhibit treatment-induced increase
Downstream Regulation
 Effect of modification (function):  enzymatic activity, induced, molecular association, regulation
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
c-Raf (rat) Induces phosphorylation, enzymatic activity, inhibited co-immunoprecipitation

S259-p - c-Raf (rat) ▲
Orthologous residues
c‑Raf (human): S259‑p, c‑Raf (mouse): S259‑p, c‑Raf (rat): S259‑p, c‑Raf (chicken): S259‑p
Characterization
 Methods used to characterize site in vivo:  phospho-antibody
 Relevant cell lines - cell types - tissues:  vascular smooth muscle cell ('muscle, smooth')
 Cellular systems studied:  cell lines
 Species studied:  rat
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE Akt1 (rat) co-immunoprecipitation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
PDGF increase
LY294002 PDGF inhibit treatment-induced increase

T203-p - ERK1 (rat) ▲
Orthologous residues
ERK1 (human): T202‑p, ERK1 (mouse): T203‑p, ERK1 (rat): T203‑p, ERK1 (hamster): T192‑p
Characterization
 Methods used to characterize site in vivo:  phospho-antibody
 Relevant cell lines - cell types - tissues:  vascular smooth muscle cell ('muscle, smooth')
 Cellular systems studied:  cell lines
 Species studied:  rat
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
PDGF increase
LY294002 PDGF no effect upon treatment-induced increase
thrombin increase
LY294002 thrombin no effect upon treatment-induced increase

Y205-p - ERK1 (rat) ▲
Orthologous residues
ERK1 (human): Y204‑p, ERK1 (mouse): Y205‑p, ERK1 (rat): Y205‑p, ERK1 (hamster): Y194‑p
Characterization
 Methods used to characterize site in vivo:  phospho-antibody
 Relevant cell lines - cell types - tissues:  vascular smooth muscle cell ('muscle, smooth')
 Cellular systems studied:  cell lines
 Species studied:  rat
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
PDGF increase
LY294002 PDGF no effect upon treatment-induced increase
thrombin increase
LY294002 thrombin no effect upon treatment-induced increase

T183-p - ERK2 (rat) ▲
Orthologous residues
ERK2 (human): T185‑p, ERK2 (mouse): T183‑p, ERK2 (rat): T183‑p, ERK2 (chicken): T193‑p
Characterization
 Methods used to characterize site in vivo:  phospho-antibody
 Relevant cell lines - cell types - tissues:  vascular smooth muscle cell ('muscle, smooth')
 Cellular systems studied:  cell lines
 Species studied:  rat
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
PDGF increase
LY294002 PDGF no effect upon treatment-induced increase
thrombin increase
LY294002 thrombin no effect upon treatment-induced increase

Y185-p - ERK2 (rat) ▲
Orthologous residues
ERK2 (human): Y187‑p, ERK2 (mouse): Y185‑p, ERK2 (rat): Y185‑p, ERK2 (chicken): Y195‑p
Characterization
 Methods used to characterize site in vivo:  phospho-antibody
 Relevant cell lines - cell types - tissues:  vascular smooth muscle cell ('muscle, smooth')
 Cellular systems studied:  cell lines
 Species studied:  rat
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
PDGF increase
LY294002 PDGF no effect upon treatment-induced increase
thrombin increase
LY294002 thrombin no effect upon treatment-induced increase

S218-p - MEK1 (rat) ▲
Orthologous residues
MEK1 (human): S218‑p, MEK1 (mouse): S218‑p, MEK1 (rat): S218‑p, MEK1 (rabbit): S218‑p
Characterization
 Methods used to characterize site in vivo:  phospho-antibody
 Cellular systems studied:  cell lines
 Species studied:  rat
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
PDGF increase
LY294002 PDGF no effect upon treatment-induced increase
thrombin increase
LY294002 thrombin no effect upon treatment-induced increase

S222-p - MEK1 (rat) ▲
Orthologous residues
MEK1 (human): S222‑p, MEK1 (mouse): S222‑p, MEK1 (rat): S222‑p, MEK1 (rabbit): S222‑p
Characterization
 Methods used to characterize site in vivo:  phospho-antibody
 Cellular systems studied:  cell lines
 Species studied:  rat


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