Curated Information

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Curated Information Page

PubMed Id: 15806160

This page summarizes selected information from the article referenced above and curated into PhosphoSitePlus^®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus^®, click here.

Information from this record has been curated, but not yet edited in PhosphoSitePlus^® and may be incomplete.

Sutherland BW, et al. (2005) Akt phosphorylates the Y-box binding protein 1 at Ser102 located in the cold shock domain and affects the anchorage-independent growth of breast cancer cells. Oncogene 24, 4281-92 15806160

Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.

S473-p - Akt1 (human)

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Orthologous residues

Akt1 (human): S473‑p, Akt1 (mouse): S473‑p, Akt1 (rat): S473‑p, Akt1 (fruit fly): S586‑p, Akt1 (cow): S473‑p

Characterization

Methods used to characterize site in vivo: phospho-antibody, western blotting

Disease tissue studied: breast cancer

Relevant cell lines - cell types - tissues: breast, MDA-MB231 (breast cell)

Cellular systems studied: cell lines, tissue

Species studied: human

Upstream Regulation

Treatments, proteins and their effect on site modification:

Treatments	Referenced Treatments	Manipulated Protein	Referenced Protein	Effect	Notes
IGF-1				increase

S102-p - YB-1 (human)

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Orthologous residues

YB‑1 (human): S102‑p, YB‑1 (mouse): S100‑p, YB‑1 (rat): S100‑p

Characterization

Methods used to characterize site in vivo: mutation of modification site

Disease tissue studied: breast cancer

Relevant cell lines - cell types - tissues: MCF-7 (breast cell)

Cellular systems studied: cell lines

Species studied: human

Enzymes shown to modify site in vitro:

Type	Enzyme
KINASE	Akt1 (human)

Upstream Regulation

Potential in vivo enzymes for site:

Type	Enzyme	Evidence	Notes
KINASE	Akt1 (human)	modification site within consensus motif, co-immunoprecipitation

Downstream Regulation

Effect of modification (function): intracellular localization

Effect of modification (process): cell growth, altered

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