Curated Information
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Curated Information Page
PubMed Id: 12766774 
This page summarizes selected information from the article referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Bulavin DV, et al. (2003) Dual phosphorylation controls Cdc25 phosphatases and mitotic entry. Nat Cell Biol 5, 545-51 12766774
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
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S307-p - CDC25B iso2 (human)
Orthologous residues
CDC25B (human): S321‑p, CDC25B iso2 (human): S307‑p, CDC25B iso3 (human): S280‑p, CDC25B (mouse): S319‑p, CDC25B (rat): S317‑p
Characterization
 Methods used to characterize site in vivo mutation of modification site, phospho-antibody
 Cellular systems studied:  cell lines
 Species studied:  human
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE CDK1 (human)

S309-p - CDC25B iso2 (human)
Orthologous residues
CDC25B (human): S323‑p, CDC25B iso2 (human): S309‑p, CDC25B iso3 (human): S282‑p, CDC25B (mouse): S321‑p, CDC25B (rat): S319‑p

S214-p - CDC25C (human)
Orthologous residues
CDC25C (human): S214‑p, CDC25C iso3 (human): S171‑p, CDC25C (mouse): , CDC25C (frog): S285‑p
Characterization
 Methods used to characterize site in vivo mutation of modification site, phospho-antibody
 Relevant cell lines - cell types - tissues:  HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  human
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE CDK1 (human)
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE CDK1 (human) pharmacological inhibitor of upstream enzyme
Downstream Regulation
 Effect of modification (function):  molecular association, regulation
 Effect of modification (process):  cell cycle regulation
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
14-3-3 beta (human) Disrupts not reported
 Comments:  In the absence of S214 phosphorylation Cdc25C can be phosphorylated on S216 and can bind to 14-3-3 proteins in mitosis

S216-p - CDC25C (human)
Orthologous residues
CDC25C (human): S216‑p, CDC25C iso3 (human): S173‑p, CDC25C (mouse): , CDC25C (frog): S287‑p
Characterization
 Methods used to characterize site in vivo mutation of modification site, phospho-antibody
 Relevant cell lines - cell types - tissues:  HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  human
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE Chk1 (human)
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
ionizing radiation no change compared to control
Downstream Regulation
 Effect of modification (function):  molecular association, regulation
 Effect of modification (process):  cell cycle regulation
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
14-3-3 beta (human) Induces co-immunoprecipitation

Y15-p - CDK1 (human)
Orthologous residues
CDK1 (human): Y15‑p, CDK1 (mouse): Y15‑p, CDK1 (rat): Y15‑p, CDK1 (chicken): Y15‑p, CDK1 (fruit fly): Y15‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody
 Relevant cell lines - cell types - tissues:  HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
ionizing radiation no change compared to control


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