Curated Information
Javascript is not enabled on this browser. This site will not work properly without Javascript.
PhosphoSitePlus Homepage Cell Signaling Technology
PhosphoSitePlus
HomeAbout PhosphoSiteUsing PhosphoSiteCuration ProcessContact
NIH-logos NIGMS Logo NIAAA Logo NCI Logo NIH Logo
Curated Information Page
PubMed Id: 15545271 
Kippenberger S, et al. (2005) Mechanical stretch stimulates protein kinase B/Akt phosphorylation in epidermal cells via angiotensin II type 1 receptor and epidermal growth factor receptor. J Biol Chem 280, 3060-7 15545271
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
Download Sites

T308-p - Akt1 (human)
Orthologous residues
Akt1 (human): T308‑p, Akt1 (mouse): T308‑p, Akt1 (rat): T308‑p, Akt1 (fruit fly): T423‑p, Akt1 (cow): T308‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  293 (epithelial), A431 (epithelial), HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
cyclic stretch increase
LY294002 cyclic stretch inhibit treatment-induced increase
wortmannin cyclic stretch inhibit treatment-induced increase
PTX cyclic stretch inhibit treatment-induced increase
AG1478 cyclic stretch inhibit treatment-induced increase
BQ-788 cyclic stretch no effect upon treatment-induced increase
BQ-123 cyclic stretch no effect upon treatment-induced increase
PD168393 cyclic stretch inhibit treatment-induced increase
losartan cyclic stretch inhibit treatment-induced increase
TNF increase
AG1478 TNF inhibit treatment-induced increase

S473-p - Akt1 (human)
Orthologous residues
Akt1 (human): S473‑p, Akt1 (mouse): S473‑p, Akt1 (rat): S473‑p, Akt1 (fruit fly): S586‑p, Akt1 (cow): S473‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  293 (epithelial), A431 (epithelial), HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
cyclic stretch increase
LY294002 cyclic stretch inhibit treatment-induced increase
wortmannin cyclic stretch inhibit treatment-induced increase
PTX cyclic stretch inhibit treatment-induced increase
PD168393 cyclic stretch inhibit treatment-induced increase
AG1478 cyclic stretch inhibit treatment-induced increase
losartan cyclic stretch inhibit treatment-induced increase
BQ-788 cyclic stretch no effect upon treatment-induced increase
BQ-123 cyclic stretch no effect upon treatment-induced increase
TNF increase
AG1478 TNF inhibit treatment-induced increase
PD168393 TNF inhibit treatment-induced increase


Home  |  Curator Login With enhanced literature mining using Linguamatics I2E I2E Logo Produced by 3rd Millennium  |  Design by Digizyme
©2003-2013 Cell Signaling Technology, Inc.