Curated Information
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Curated Information Page
PubMed Id: 19015317 
This page summarizes selected information from the article referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Huang H, et al. (2008) Phosphorylation sites in BubR1 that regulate kinetochore attachment, tension, and mitotic exit. J Cell Biol 183, 667-80 19015317
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
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S435-p - BubR1 (human) ▲
Orthologous residues
BubR1 (human): S435‑p, BubR1 (mouse): S428‑p, BubR1 (rat): S428‑p
Characterization
 Methods used to characterize site in vivo:  immunoprecipitation, mass spectrometry, mutation of modification site, phospho-antibody
 Relevant cell lines - cell types - tissues:  HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE TTK (human) siRNA inhibition of enzyme
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
nocodazole increase
hesperadin nocodazole no effect upon treatment-induced increase
roscovitine nocodazole no effect upon treatment-induced increase
staurosporine nocodazole no effect upon treatment-induced increase

S543-p - BubR1 (human) ▲
Orthologous residues
BubR1 (human): S543‑p, BubR1 (mouse): S535‑p, BubR1 (rat): S535‑p
Characterization
 Methods used to characterize site in vivo:  immunoprecipitation, mass spectrometry, mutation of modification site, phospho-antibody
 Relevant cell lines - cell types - tissues:  HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE TTK (human) siRNA inhibition of enzyme
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
nocodazole increase
hesperadin nocodazole no effect upon treatment-induced increase
roscovitine nocodazole no effect upon treatment-induced increase
staurosporine nocodazole no effect upon treatment-induced increase

S670-p - BubR1 (human) ▲
Orthologous residues
BubR1 (human): S670‑p, BubR1 (mouse): S659‑p, BubR1 (rat): S659‑p
Characterization
 Methods used to characterize site in vivo:  immunoprecipitation, mass spectrometry, mutation of modification site, phospho-antibody
 Relevant cell lines - cell types - tissues:  HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE TTK (human) siRNA inhibition of enzyme
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
nocodazole increase
hesperadin nocodazole no effect upon treatment-induced increase
roscovitine nocodazole no effect upon treatment-induced increase
staurosporine nocodazole no effect upon treatment-induced increase
Downstream Regulation
 Effect of modification (process):  cell cycle regulation
 Comments:  phosphorylated at kinetochores at the onset of mitosis; pS670 Ab delayed anaphase onset in vivo and in vitro;

S1043-p - BubR1 (human) ▲
Orthologous residues
BubR1 (human): S1043‑p, BubR1 (mouse): S1033‑p, BubR1 (rat): S1033‑p
Characterization
 Methods used to characterize site in vivo:  immunoprecipitation, mass spectrometry, mutation of modification site, phospho-antibody
 Relevant cell lines - cell types - tissues:  HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE TTK (human) siRNA inhibition of enzyme
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
nocodazole increase
hesperadin nocodazole no effect upon treatment-induced increase
roscovitine nocodazole no effect upon treatment-induced increase
staurosporine nocodazole no effect upon treatment-induced increase
Downstream Regulation
 Effect of modification (process):  cell cycle regulation
 Comments:  pS1043 Ab delayed anaphase onset in vivo and in vitro


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