Curated Information
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Curated Information Page
PubMed Id: 18695677 
Tang J, Yang X, Liu X (2008) Phosphorylation of Plk1 at Ser326 regulates its functions during mitotic progression. Oncogene 27, 6635-45 18695677
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Click on the protein name to open the protein page, and on the RSD number to open the site page.
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S326-p - PLK1 (human)
Orthologous residues
PLK1 (human): S326‑p, PLK1 (mouse): S326‑p, PLK1 (rat): S326‑p, PLK1 (frog): S317‑p
 Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  human
 Enzymes shown to modify site in vitro
Type Enzyme
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE MAPKAPK2 (human) transfection of wild-type enzyme, genetic knockout/knockin of upstream enzyme
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
roscovitine increase
nocodazole increase
Downstream Regulation
 Effect of modification (function):  enzymatic activity, induced
 Effect of modification (process):  cell cycle regulation

S383-p - PLK1 (human)
Orthologous residues
PLK1 (human): S383‑p, PLK1 (mouse): S383‑p, PLK1 (rat): S383‑p, PLK1 (frog): C377‑p
 Enzymes shown to modify site in vitro
Type Enzyme

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