Curated Information
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PubMed Id: 15159397 
This page summarizes selected information from the article referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Fabbro M, et al. (2004) BRCA1-BARD1 complexes are required for p53Ser-15 phosphorylation and a G1/S arrest following ionizing radiation-induced DNA damage. J Biol Chem 279, 31251-8 15159397
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
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S1981-p - ATM (human) ▲
Orthologous residues
ATM (human): S1981‑p, ATM (mouse): S1987‑p
Characterization
 Methods used to characterize site in vivo:  phospho-antibody
 Relevant cell lines - cell types - tissues:  293T (epithelial), HCC1937 (breast cell), MCF-7 (breast cell), U2OS (bone cell) [GR (human)]
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
ionizing radiation increase
UV no change compared to control

S1423-p - BRCA1 (human) ▲
Orthologous residues
BRCA1 (human): S1423‑p, BRCA1 iso6 (human): S320‑p, BRCA1 (mouse): N1379‑p, BRCA1 (rat): S1380‑p
Characterization
 Methods used to characterize site in vivo:  phospho-antibody
 Relevant cell lines - cell types - tissues:  293T (epithelial), HCC1937 (breast cell), MCF-7 (breast cell), U2OS (bone cell) [GR (human)]
 Cellular systems studied:  cell lines
 Species studied:  human
Downstream Regulation
 Effect of modification (function):  phosphorylation
 Comments:  phosphorylation of BRCA1 at S1423 and S1524 is required for ATM/ATR-mediated p53 S15 phosphorylation.

S1524-p - BRCA1 (human) ▲
Orthologous residues
BRCA1 (human): S1524‑p, BRCA1 iso6 (human): S420‑p, BRCA1 (mouse): S1481‑p, BRCA1 (rat): S1482‑p
Characterization
 Methods used to characterize site in vivo:  phospho-antibody
 Relevant cell lines - cell types - tissues:  293T (epithelial), HCC1937 (breast cell), MCF-7 (breast cell), U2OS (bone cell) [GR (human)]
 Cellular systems studied:  cell lines
 Species studied:  human
Downstream Regulation
 Effect of modification (function):  phosphorylation
 Comments:  phosphorylation of BRCA1 at S1423 and S1524 is required for ATM/ATR-mediated p53 S15 phosphorylation.

S317-p - Chk1 (human) ▲
Orthologous residues
Chk1 (human): S317‑p, Chk1 (mouse): S317‑p, Chk1 (rat): S317‑p, Chk1 (chicken): S317‑p
Characterization
 Methods used to characterize site in vivo:  phospho-antibody
 Relevant cell lines - cell types - tissues:  293T (epithelial), HCC1937 (breast cell), MCF-7 (breast cell), U2OS (bone cell) [GR (human)]
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
UV increase
ionizing radiation increase

T68-p - Chk2 (human) ▲
Orthologous residues
Chk2 (human): T68‑p, Chk2 iso12 (human): T68‑p, Chk2 (mouse): T77‑p, Chk2 (rat): T76‑p
Characterization
 Methods used to characterize site in vivo:  phospho-antibody
 Relevant cell lines - cell types - tissues:  293T (epithelial), HCC1937 (breast cell), MCF-7 (breast cell), U2OS (bone cell) [GR (human)]
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
ionizing radiation increase
UV no change compared to control

S140-p - H2AX (human) ▲
Orthologous residues
H2AX (human): S140‑p, H2AX (mouse): S140‑p, H2AX (rat): S140‑p
Characterization
 Methods used to characterize site in vivo:  phospho-antibody
 Relevant cell lines - cell types - tissues:  293T (epithelial), HCC1937 (breast cell), MCF-7 (breast cell), U2OS (bone cell) [GR (human)]
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
ionizing radiation increase
UV increase

S63-p - Jun (human) ▲
Orthologous residues
Jun (human): S63‑p, Jun (mouse): S63‑p, Jun (rat): S63‑p
Characterization
 Methods used to characterize site in vivo:  phospho-antibody
 Relevant cell lines - cell types - tissues:  293T (epithelial), HCC1937 (breast cell), MCF-7 (breast cell), U2OS (bone cell) [GR (human)]
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
ionizing radiation no change compared to control
UV increase

S15-p - p53 (human) ▲
Orthologous residues
p53 (human): S15‑p, p53 (mouse): S15‑p, p53 iso2 (mouse): S18‑p, p53 (rat): S15‑p, p53 (rabbit): S15‑p, p53 (monkey): S15‑p
Characterization
 Methods used to characterize site in vivo:  phospho-antibody
 Relevant cell lines - cell types - tissues:  293T (epithelial), HCC1937 (breast cell), MCF-7 (breast cell), U2OS (bone cell) [GR (human)]
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
ionizing radiation BRCA1 (human), BARD1 (human) increase
UV BRCA1 (human), BARD1 (human) increase
Downstream Regulation
 Effect of modification (process):  cell cycle regulation


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