|
Orthologous residues
|
|
STXBP4 (human): S99‑p, STXBP4 iso4 (human): S24‑p, STXBP4 (mouse): S99‑p
|
|
Characterization
|
|
Methods used to characterize site in vivo:
mutation of modification site, phospho-antibody
|
|
Cellular systems studied:
cell lines
|
|
Species studied:
hamster
|
|
Enzymes shown to modify site in vitro:
|
|
|
|
Upstream Regulation
|
|
Potential in vivo enzymes for site:
|
|
Type
|
Enzyme
|
Evidence
|
Notes
|
|
KINASE
|
Akt2 (mouse)
|
transfection of wild-type enzyme, co-immunoprecipitation
|
|
|
|
Treatments, proteins and their effect on site modification:
|
|
Treatments
|
Referenced Treatments
|
Manipulated Protein
|
Referenced Protein
|
Effect
|
Notes
|
|
insulin
|
|
|
|
increase
|
|
|
|
Downstream Regulation
|
|
Effect of modification (function):
molecular association, regulation
|
|
Modification regulates interactions with:
|
|
Interacting molecule
|
Interacting domains
|
Effect
|
Consequences (function)
|
Consequences (process)
|
Detection assays
|
|
GLUT4 (mouse)
|
|
Disrupts
|
intracellular localization
|
|
co-immunoprecipitation, electrophoretic visualization
|
|
STX4 (mouse)
|
|
Disrupts
|
intracellular localization
|
|
co-immunoprecipitation, electrophoretic visualization
|
|
