Curated Information
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Curated Information Page
PubMed Id: 15775972 
This page summarizes selected information from the article referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Riento K, et al. (2005) RhoE function is regulated by ROCK I-mediated phosphorylation. EMBO J 24, 1170-80 15775972
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
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S7-p - RhoE (human) ▲
Orthologous residues
RhoE (human): S7‑p, RhoE (mouse): S7‑p
Characterization
 Methods used to characterize site in vivo:  [32P] bio-synthetic labeling, electrophoretic mobility shift, mutation of modification site, phospho-antibody
 Relevant cell lines - cell types - tissues:  3T3 (fibroblast) [SHP-2 (mouse), homozygous knockout], COS (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  monkey, mouse
 Enzymes shown to modify site in vitro: 
Type Enzyme
KINASE ROCK1 (human)
PHOSPHATASE PPP1CA (human)
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
PDGF no change compared to control
Y27632 decrease

S11-p - RhoE (human) ▲
Orthologous residues
RhoE (human): S11‑p, RhoE (mouse): S11‑p
Characterization
 Methods used to characterize site in vivo:  [32P] bio-synthetic labeling, electrophoretic mobility shift, mutation of modification site, phospho-antibody
 Relevant cell lines - cell types - tissues:  3T3 (fibroblast) [SHP-2 (mouse), homozygous knockout], COS (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  monkey, mouse
 Enzymes shown to modify site in vitro: 
Type Enzyme
KINASE ROCK1 (human)
PHOSPHATASE PPP1CA (human)
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE ROCK1 (human) pharmacological inhibitor of upstream enzyme, transfection of wild-type enzyme
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
PDGF increase
Y27632 PDGF inhibit treatment-induced increase
H1152 PDGF inhibit treatment-induced increase
U0126 PDGF no effect upon treatment-induced increase
LY294002 PDGF no effect upon treatment-induced increase
GF109203X PDGF inhibit treatment-induced increase
phorbol ester increase
Y27632 phorbol ester inhibit treatment-induced increase
H1152 phorbol ester inhibit treatment-induced increase
GF109203X phorbol ester inhibit treatment-induced increase
LPA no change compared to control
Downstream Regulation
 Effect of modification (function):  intracellular localization, protein stabilization


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