Curated Information
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Curated Information Page
PubMed Id: 18951975 
This page summarizes selected information from the article referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Wei Z, et al. (2009) Haloperidol disrupts Akt signalling to reveal a phosphorylation-dependent regulation of pro-apoptotic Bcl-XS function. Cell Signal 21, 161-8 18951975
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
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T308-p - Akt1 (rat)
Orthologous residues
Akt1 (human): T308‑p, Akt1 (mouse): T308‑p, Akt1 (rat): T308‑p, Akt1 (fruit fly): T423‑p, Akt1 (cow): T308‑p
Characterization
 Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, phospho-antibody, western blotting
 Disease tissue studied:  adrenal cancer, pheochromocytoma
 Relevant cell lines - cell types - tissues:  293A (epithelial), neuron, PC-12 (chromaffin)
 Cellular systems studied:  cell lines, primary cells
 Species studied:  human, rat
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
haloperidol decrease

S473-p - Akt1 (rat)
Orthologous residues
Akt1 (human): S473‑p, Akt1 (mouse): S473‑p, Akt1 (rat): S473‑p, Akt1 (fruit fly): S586‑p, Akt1 (cow): S473‑p
Characterization
 Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, phospho-antibody, western blotting
 Disease tissue studied:  adrenal cancer, pheochromocytoma
 Relevant cell lines - cell types - tissues:  293A (epithelial), neuron, PC-12 (chromaffin)
 Cellular systems studied:  cell lines, primary cells
 Species studied:  human, rat
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
haloperidol decrease
DTG no change compared to control
LY294002 decrease

S137-p - BAD (rat)
Orthologous residues
BAD (human): S99‑p, BAD (mouse): S136‑p, BAD (rat): S137‑p
Characterization
 Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, phospho-antibody, western blotting
 Disease tissue studied:  adrenal cancer, pheochromocytoma
 Relevant cell lines - cell types - tissues:  293A (epithelial), neuron, PC-12 (chromaffin)
 Cellular systems studied:  cell lines, primary cells
 Species studied:  human, rat
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
haloperidol decrease

S62-p - Bcl-xL (rat)
Orthologous residues
Bcl‑xL (human): S62‑p, Bcl‑xL (mouse): S62‑p, Bcl‑xL (rat): S62‑p
Characterization
 Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, phospho-antibody, western blotting
 Disease tissue studied:  adrenal cancer, pheochromocytoma
 Relevant cell lines - cell types - tissues:  293A (epithelial), neuron, PC-12 (chromaffin)
 Cellular systems studied:  cell lines, primary cells
 Species studied:  human, rat
Downstream Regulation
 Effect of modification (process):  apoptosis, altered

S106-p - Bcl-xL (rat)
Orthologous residues
Bcl‑xL (human): S106‑p, Bcl‑xL (mouse): S106‑p, Bcl‑xL (rat): S106‑p
Characterization
 Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, phospho-antibody, western blotting
 Disease tissue studied:  adrenal cancer, pheochromocytoma
 Relevant cell lines - cell types - tissues:  293A (epithelial), neuron, PC-12 (chromaffin)
 Cellular systems studied:  cell lines, primary cells
 Species studied:  human, rat
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE Akt1 (rat) transfection of constitutively active enzyme, modification site within consensus motif, co-immunoprecipitation, phospho-motif antibody
Downstream Regulation
 Effect of modification (function):  molecular association, regulation
 Effect of modification (process):  apoptosis, altered
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
Akt1 (rat) Induces co-immunoprecipitation
VDAC1 (rat) Disrupts co-immunoprecipitation

S228-p - Bcl-xL (rat)
Orthologous residues
Bcl‑xL (human): S228‑p, Bcl‑xL (mouse): S228‑p, Bcl‑xL (rat): S228‑p
Characterization
 Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, phospho-antibody, western blotting
 Disease tissue studied:  adrenal cancer, pheochromocytoma
 Relevant cell lines - cell types - tissues:  293A (epithelial), neuron, PC-12 (chromaffin)
 Cellular systems studied:  cell lines, primary cells
 Species studied:  human, rat
Downstream Regulation
 Effect of modification (process):  apoptosis, altered


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