Curated Information
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Curated Information Page
PubMed Id: 18922800 
This page summarizes selected information from the article referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Chi YH, et al. (2008) Requirements for protein phosphorylation and the kinase activity of polo-like kinase 1 (Plk1) for the kinetochore function of mitotic arrest deficiency protein 1 (Mad1). J Biol Chem 283, 35834-44 18922800
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
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S22-p - MAD1L1 (human)
Orthologous residues
MAD1L1 (human): S22‑p, MAD1L1 iso3 (human): S22‑p, MAD1L1 iso4 (human): , MAD1L1 (mouse): S22‑p
Characterization
 Methods used to characterize site in vivo immunoprecipitation, mass spectrometry, mutation of modification site
 Relevant cell lines - cell types - tissues:  HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE PLK1 (human) co-immunoprecipitation, transfection of wild-type enzyme

S29-p - MAD1L1 (human)
Orthologous residues
MAD1L1 (human): S29‑p, MAD1L1 iso3 (human): S29‑p, MAD1L1 iso4 (human): , MAD1L1 (mouse): S29‑p
Characterization
 Methods used to characterize site in vivo immunoprecipitation, mass spectrometry, mutation of modification site
 Relevant cell lines - cell types - tissues:  HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE PLK1 (human) co-immunoprecipitation, transfection of wild-type enzyme

S428-p - MAD1L1 (human)
Orthologous residues
MAD1L1 (human): S428‑p, MAD1L1 iso3 (human): S427‑p, MAD1L1 iso4 (human): , MAD1L1 (mouse): S428‑p
Characterization
 Methods used to characterize site in vivo immunoprecipitation, mass spectrometry, mutation of modification site
 Relevant cell lines - cell types - tissues:  HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  human

T680-p - MAD1L1 (human)
Orthologous residues
MAD1L1 (human): T680‑p, MAD1L1 iso3 (human): , MAD1L1 iso4 (human): T231‑p, MAD1L1 (mouse): T679‑p
Characterization
 Methods used to characterize site in vivo immunoprecipitation, mass spectrometry, mutation of modification site
 Relevant cell lines - cell types - tissues:  HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE PLK1 (human) co-immunoprecipitation, transfection of wild-type enzyme
Downstream Regulation
 Effect of modification (function):  activity, induced, intracellular localization
 Effect of modification (process):  cell cycle regulation

S699-p - MAD1L1 (human)
Orthologous residues
MAD1L1 (human): S699‑p, MAD1L1 iso3 (human): , MAD1L1 iso4 (human): S250‑p, MAD1L1 (mouse): S698‑p
Characterization
 Methods used to characterize site in vivo immunoprecipitation, mass spectrometry, mutation of modification site
 Relevant cell lines - cell types - tissues:  HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  human

T708-p - MAD1L1 (human)
Orthologous residues
MAD1L1 (human): T708‑p, MAD1L1 iso3 (human): , MAD1L1 iso4 (human): T259‑p, MAD1L1 (mouse): T707‑p
Characterization
 Methods used to characterize site in vivo immunoprecipitation, mass spectrometry, mutation of modification site
 Relevant cell lines - cell types - tissues:  HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  human
Downstream Regulation
 Effect of modification (function):  intracellular localization


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