Curated Information
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Curated Information Page
PubMed Id: 18838540 
This page summarizes selected information from the article referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Galliher-Beckley AJ, Williams JG, Collins JB, Cidlowski JA (2008) Glycogen Synthase Kinase 3{beta}-Mediated Serine Phosphorylation of the Human Glucocorticoid Receptor Redirects Gene Expression Profiles. Mol Cell Biol 28, 7309-22 18838540
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
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S211-p - GR (human) ▲
Orthologous residues
GR (human): S211‑p, GR iso2 (human): S211‑p, GR (mouse): S220‑p, GR (rat): S232‑p
Characterization
 Methods used to characterize site in vivo:  phospho-antibody, western blotting
 Disease tissue studied:  bone cancer
 Relevant cell lines - cell types - tissues:  U2OS (bone cell)
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
dexamethasone increase
GSK-3 inhibitor X dexamethasone no effect upon treatment-induced increase slight inhibition

S404-p - GR (human) ▲
Orthologous residues
GR (human): S404‑p, GR iso2 (human): S404‑p, GR (mouse): S412‑p, GR (rat): S424‑p
Characterization
 Methods used to characterize site in vivo:  mass spectrometry, mutation of modification site, phospho-antibody, western blotting
 Disease tissue studied:  bone cancer, liver cancer, lung cancer
 Relevant cell lines - cell types - tissues:  A549 (pulmonary), HeLa (cervical), HepG2 (hepatic), MG63 (bone cell), U2OS (bone cell)
 Cellular systems studied:  cell lines
 Species studied:  human
 Enzymes shown to modify site in vitro: 
Type Enzyme
KINASE GSK3B (human)
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE GSK3B (human) pharmacological activator of upstream enzyme, modification site within consensus motif, transfection of constitutively active enzyme, co-immunoprecipitation, genetic knockout/knockin of upstream enzyme, pharmacological inhibitor of upstream enzyme, phospho-antibody, transfection of inactive enzyme
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
dexamethasone increase
GSK-3 inhibitor X dexamethasone inhibit treatment-induced increase
Downstream Regulation
 Effect of modification (function):  inhibition, intracellular localization, molecular association, regulation, protein conformation, protein degradation
 Effect of modification (process):  apoptosis, inhibited, transcription, altered
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
NFkB-p65 (human) Disrupts co-immunoprecipitation
p300 (human) Induces co-immunoprecipitation

S220-p - GR (mouse) ▲
Orthologous residues
GR (human): S211‑p, GR iso2 (human): S211‑p, GR (mouse): S220‑p, GR (rat): S232‑p
Characterization
 Methods used to characterize site in vivo:  phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  MEF (fibroblast), MEF (fibroblast) [GSK3B (mouse), homozygous knockout]
 Cellular systems studied:  cell lines
 Species studied:  mouse
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
dexamethasone increase

S412-p - GR (mouse) ▲
Orthologous residues
GR (human): S404‑p, GR iso2 (human): S404‑p, GR (mouse): S412‑p, GR (rat): S424‑p
Characterization
 Methods used to characterize site in vivo:  phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  MEF (fibroblast), MEF (fibroblast) [GSK3B (mouse), homozygous knockout]
 Cellular systems studied:  cell lines
 Species studied:  mouse
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE GSK3B (human) pharmacological activator of upstream enzyme, modification site within consensus motif, transfection of constitutively active enzyme, co-immunoprecipitation, genetic knockout/knockin of upstream enzyme, pharmacological inhibitor of upstream enzyme, phospho-antibody, transfection of inactive enzyme
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
dexamethasone increase


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