Curated Information
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Curated Information Page
PubMed Id: 10673501 
This page summarizes selected information from the article referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Shieh SY, et al. (2000) The human homologs of checkpoint kinases Chk1 and Cds1 (Chk2) phosphorylate p53 at multiple DNA damage-inducible sites. Genes Dev 14, 289-300 10673501
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
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S15-p - p53 (human) ▲
Orthologous residues
p53 (human): S15‑p, p53 (mouse): S15‑p, p53 iso2 (mouse): S18‑p, p53 (rat): S15‑p, p53 (rabbit): S15‑p, p53 (monkey): S15‑p
Characterization
 Methods used to characterize site in vivo:  phospho-antibody, western blotting
 Disease tissue studied:  lung cancer, non-small cell lung cancer
 Relevant cell lines - cell types - tissues:  NCI-H1299 (pulmonary)
 Cellular systems studied:  cell lines
 Species studied:  human
 Enzymes shown to modify site in vitro: 
Type Enzyme
KINASE Chk2 (human)
KINASE Chk1 (human)
KINASE DNA-PK (human)
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE Chk1 (human) transfection of inactive enzyme, antisense inhibition of upstream enzyme
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
ionizing radiation increase

T18-p - p53 (human) ▲
Orthologous residues
p53 (human): T18‑p, p53 (mouse): T18‑p, p53 iso2 (mouse): T21‑p, p53 (rat): T18‑p, p53 (rabbit): T18‑p, p53 (monkey): T18‑p
Characterization
 Enzymes shown to modify site in vitro: 
Type Enzyme
KINASE Chk2 (human)

S20-p - p53 (human) ▲
Orthologous residues
p53 (human): S20‑p, p53 (mouse): S20‑p, p53 iso2 (mouse): S23‑p, p53 (rat): S20‑p, p53 (rabbit): S20‑p, p53 (monkey): S20‑p
Characterization
 Enzymes shown to modify site in vitro: 
Type Enzyme
KINASE Chk2 (human)
KINASE Chk1 (human)

S37-p - p53 (human) ▲
Orthologous residues
p53 (human): S37‑p, p53 (mouse): S34‑p, p53 iso2 (mouse): S37‑p, p53 (rat): S39‑p, p53 (rabbit): N37‑p, p53 (monkey): S37‑p
Characterization
 Enzymes shown to modify site in vitro: 
Type Enzyme
KINASE Chk1 (human)
KINASE Chk2 (human)


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