Curated Information
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Curated Information Page
PubMed Id: 11673466 
This page summarizes selected information from the article referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Miyazaki K, et al. (2002) Rho-dependent agonist-induced spatio-temporal change in myosin phosphorylation in smooth muscle cells. J Biol Chem 277, 725-34 11673466
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S20-p - MRLC1 (human)
Orthologous residues
MRLC1 (human): S20‑p, MRLC1 (mouse): S20‑p, MRLC1 (rat): S20‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  'muscle, smooth', trachea
 Cellular systems studied:  primary cells
 Species studied:  pig
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
carbachol increase
carbachol RhoA (human) inhibit treatment-induced increase DN RhoA
carbachol RhoA (human) augment treatment-induced increase wt
carbachol RhoA (human) augment treatment-induced increase activated
Y27632 carbachol RhoA (human) inhibit treatment-induced increase activated RhoA
ML-9 carbachol inhibit treatment-induced increase


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