Curated Information
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Curated Information Page
PubMed Id: 10995739 
This page summarizes selected information from the article referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Zheng WH, Kar S, Quirion R (2000) Insulin-like growth factor-1-induced phosphorylation of the forkhead family transcription factor FKHRL1 is mediated by Akt kinase in PC12 cells. J Biol Chem 275, 39152-8 10995739
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
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T32-p - FOXO3A (human)
Orthologous residues
FOXO3A (human): T32‑p, FOXO3A (mouse): T32‑p, FOXO3A (rat): T32‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Disease tissue studied:  adrenal cancer, pheochromocytoma
 Relevant cell lines - cell types - tissues:  293 (epithelial), PC-12 (chromaffin)
 Cellular systems studied:  cell lines
 Species studied:  human, rat
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE Akt1 (human)
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE Akt1 (rat) phospho-motif antibody, co-immunoprecipitation, transfection of dominant-negative enzyme, pharmacological activator of upstream enzyme, pharmacological inhibitor of upstream enzyme, phospho-antibody, transfection of constitutively active enzyme

S253-p - FOXO3A (human)
Orthologous residues
FOXO3A (human): S253‑p, FOXO3A (mouse): S252‑p, FOXO3A (rat): S252‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Disease tissue studied:  adrenal cancer, pheochromocytoma
 Relevant cell lines - cell types - tissues:  293 (epithelial), PC-12 (chromaffin)
 Cellular systems studied:  cell lines
 Species studied:  human, rat
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE Akt1 (human)
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE Akt1 (rat) phospho-motif antibody, co-immunoprecipitation, transfection of dominant-negative enzyme, pharmacological activator of upstream enzyme, pharmacological inhibitor of upstream enzyme, phospho-antibody, transfection of constitutively active enzyme

T32-p - FOXO3A (rat)
Orthologous residues
FOXO3A (human): T32‑p, FOXO3A (mouse): T32‑p, FOXO3A (rat): T32‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Disease tissue studied:  adrenal cancer, pheochromocytoma
 Relevant cell lines - cell types - tissues:  293 (epithelial), PC-12 (chromaffin)
 Cellular systems studied:  cell lines
 Species studied:  human, rat
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE Akt1 (human)
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE Akt1 (rat) phospho-motif antibody, co-immunoprecipitation, transfection of dominant-negative enzyme, pharmacological activator of upstream enzyme, pharmacological inhibitor of upstream enzyme, phospho-antibody, transfection of constitutively active enzyme
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
IGF-1 increase
wortmannin IGF-1 inhibit treatment-induced increase
LY294002 IGF-1 inhibit treatment-induced increase
PD98059 IGF-1 no effect upon treatment-induced increase
rapamycin IGF-1 no effect upon treatment-induced increase

S252-p - FOXO3A (rat)
Orthologous residues
FOXO3A (human): S253‑p, FOXO3A (mouse): S252‑p, FOXO3A (rat): S252‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Disease tissue studied:  adrenal cancer, pheochromocytoma
 Relevant cell lines - cell types - tissues:  293 (epithelial), PC-12 (chromaffin)
 Cellular systems studied:  cell lines
 Species studied:  human, rat
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE Akt1 (human)
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE Akt1 (rat) phospho-motif antibody, co-immunoprecipitation, transfection of dominant-negative enzyme, pharmacological activator of upstream enzyme, pharmacological inhibitor of upstream enzyme, phospho-antibody, transfection of constitutively active enzyme
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
IGF-1 increase
wortmannin IGF-1 inhibit treatment-induced increase
LY294002 IGF-1 inhibit treatment-induced increase
PD98059 IGF-1 no effect upon treatment-induced increase
rapamycin IGF-1 no effect upon treatment-induced increase


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