Boutahar N, Guignandon A, Vico L, Lafage-Proust MH (2004) Mechanical strain on osteoblasts activates autophosphorylation of focal adhesion kinase and proline-rich tyrosine kinase 2 tyrosine sites involved in ERK activation. J Biol Chem 279, 30588-99 15096502

This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.

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Y185-p - ERK2 (rat) ▲

Modsite: HtGFLtEyVAtRWYR SwissProt Entrez-Gene Orthologous residues ERK2 (human): Y187‑p, ERK2 (mouse): Y185‑p, ERK2 (rat): Y185‑p, ERK2 (chicken): Y195‑p, ERK2 (cow): Y187‑p Characterization Methods used to characterize site in vivo:  phospho-antibody, western blotting Disease tissue studied:  bone cancer Relevant cell lines - cell types - tissues:  ROS 17/2.8 (bone cell) Cellular systems studied:  cell lines Species studied:  rat Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes mechanical stress increase

Y397-p - FAK (rat) ▲

Modsite: SVSETDDyAEIIDEE SwissProt Entrez-Gene Orthologous residues FAK (human): Y397‑p, FAK iso2 (human): Y216‑p, FAK iso5 (human): Y397‑p, FAK (mouse): Y397‑p, FAK iso2 (mouse): Y428‑p, FAK iso4 (mouse): Y397‑p, FAK iso9 (mouse): ‑, FAK (rat): Y397‑p, FAK (chicken): Y397‑p, FAK iso5 (chicken): ‑ Characterization Methods used to characterize site in vivo:  phospho-antibody, western blotting Disease tissue studied:  bone cancer Relevant cell lines - cell types - tissues:  MC3T3-E1 (preosteoblast), osteoblast-calvarium, ROS 17/2.8 (bone cell) Cellular systems studied:  cell lines Species studied:  mouse, rat Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes mechanical stress increase PP2 mechanical stress inhibit treatment-induced increase PP2 decrease

Y928-p - FAK (rat) ▲

Modsite: DRSNDKVyENVTGLV SwissProt Entrez-Gene Orthologous residues FAK (human): Y925‑p, FAK iso2 (human): Y752‑p, FAK iso5 (human): Y938‑p, FAK (mouse): Y925‑p, FAK iso2 (mouse): Y956‑p, FAK iso4 (mouse): Y928‑p, FAK iso9 (mouse): Y233‑p, FAK (rat): Y928‑p, FAK (chicken): Y926‑p, FAK iso5 (chicken): Y232‑p Characterization Methods used to characterize site in vivo:  phospho-antibody, western blotting Disease tissue studied:  bone cancer Relevant cell lines - cell types - tissues:  osteoblast-calvarium, ROS 17/2.8 (bone cell) Cellular systems studied:  cell lines Species studied:  rat Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes mechanical stress increase

Y402-p - Pyk2 (rat) ▲

Modsite: CSIESDIyAEIPDET SwissProt Entrez-Gene Orthologous residues Pyk2 (human): Y402‑p, Pyk2 iso2 (human): Y402‑p, Pyk2 (mouse): Y402‑p, Pyk2 (rat): Y402‑p Characterization Methods used to characterize site in vivo:  mutation of modification site, phospho-antibody, western blotting Disease tissue studied:  bone cancer Relevant cell lines - cell types - tissues:  ROS 17/2.8 (bone cell) Cellular systems studied:  cell lines Species studied:  rat Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes mechanical stress increase thapsigargin mechanical stress inhibit treatment-induced increase EGTA mechanical stress inhibit treatment-induced increase BAPTA-AM mechanical stress inhibit treatment-induced increase Downstream Regulation Effect of modification (function):  molecular association, regulation Modification regulates interactions with:  Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays FAK (rat) Induces co-immunoprecipitation

Y419-p - Src (rat) ▲

Modsite: RLIEDNEyTARQGAk SwissProt Entrez-Gene Orthologous residues Src (human): Y419‑p, Src iso2 (human): Y425‑p, Src (mouse): Y418‑p, Src iso1 (mouse): Y424‑p, Src (rat): Y419‑p, Src (chicken): Y416‑p Characterization Methods used to characterize site in vivo:  phospho-antibody, western blotting Disease tissue studied:  bone cancer Relevant cell lines - cell types - tissues:  ROS 17/2.8 (bone cell) Cellular systems studied:  cell lines Species studied:  rat Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes mechanical stress increase