|
Orthologous residues
|
|
WASP (human): Y291‑p, WASP (mouse): Y293‑p
|
|
Characterization
|
|
Methods used to characterize site in vivo:
mutation of modification site, phospho-antibody
|
|
Relevant cell lines - cell types - tissues:
Jurkat (T lymphocyte), T lymphocyte-blood
|
|
Cellular systems studied:
cell lines, primary cells
|
|
Species studied:
human, mouse
|
|
Enzymes shown to modify site in vitro:
|
|
|
|
Upstream Regulation
|
|
Potential in vivo enzymes for site:
|
|
Type
|
Enzyme
|
Evidence
|
Notes
|
|
KINASE
|
Fyn (mouse)
|
transfection of wild-type enzyme, co-immunoprecipitation, genetic knockout/knockin of upstream enzyme, transfection of dominant-negative enzyme
|
|
|
PHOSPHATASE
|
PTP-PEST (mouse)
|
transfection of wild-type enzyme, transfection of inactive enzyme
|
|
|
|
Treatments, proteins and their effect on site modification:
|
|
Treatments
|
Referenced Treatments
|
Manipulated Protein
|
Referenced Protein
|
Effect
|
Notes
|
|
anti-CD28
|
|
|
|
increase
|
|
|
anti-CD3
|
|
|
|
increase
|
|
|
|
Downstream Regulation
|
|
Effect of modification (function):
activation
|
|
Effect of modification (process):
cytoskeletal reorganization
|
