Curated Information

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PubMed Id: 18808171

This page summarizes selected information from the article referenced above and curated into PhosphoSitePlus^®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus^®, click here.

Cenni V, et al. (2008) Lamin A Ser404 is a nuclear target of Akt phosphorylation in C2C12 cells. J Proteome Res 7, 4727-35 18808171

Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.

S404-p - lamin A/C (human)

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Orthologous residues

lamin A/C (human): S404‑p, lamin A/C iso2 (human): S404‑p, lamin A/C (mouse): S404‑p, lamin A/C iso3 (mouse): S292‑p, lamin A/C (rat): S404‑p

Characterization

Methods used to characterize site in vivo: 2D analysis, mutation of modification site, phospho-antibody, western blotting

Disease tissue studied: Emery-Dreifuss muscular dystrophy

Relevant cell lines - cell types - tissues: C2C12 (myoblast), fibroblast

Cellular systems studied: cell lines, primary cells

Species studied: human, mouse

Associated Diseases

Diseases:	Alterations:	Comments:
Emery-Dreifuss muscular dystrophy	mutation of site

S404-p - lamin A/C (mouse)

▲

Orthologous residues

lamin A/C (human): S404‑p, lamin A/C iso2 (human): S404‑p, lamin A/C (mouse): S404‑p, lamin A/C iso3 (mouse): S292‑p, lamin A/C (rat): S404‑p

Characterization

Methods used to characterize site in vivo: 2D analysis, mutation of modification site, phospho-antibody, western blotting

Disease tissue studied: Emery-Dreifuss muscular dystrophy

Relevant cell lines - cell types - tissues: C2C12 (myoblast), fibroblast

Cellular systems studied: cell lines, primary cells

Species studied: human, mouse

S301-p - lamin A/C (rat)

▲

Orthologous residues

lamin A/C (human): S301‑p, lamin A/C iso2 (human): S301‑p, lamin A/C (mouse): S301‑p, lamin A/C iso3 (mouse): S189‑p, lamin A/C (rat): S301‑p

Characterization

Enzymes shown to modify site in vitro:

Type	Enzyme
KINASE	Akt1 (human)

S404-p - lamin A/C (rat)

▲

Orthologous residues

lamin A/C (human): S404‑p, lamin A/C iso2 (human): S404‑p, lamin A/C (mouse): S404‑p, lamin A/C iso3 (mouse): S292‑p, lamin A/C (rat): S404‑p

Characterization

Methods used to characterize site in vivo: 2D analysis, mutation of modification site, phospho-antibody, western blotting

Disease tissue studied: Emery-Dreifuss muscular dystrophy

Relevant cell lines - cell types - tissues: C2C12 (myoblast), fibroblast

Cellular systems studied: cell lines, primary cells

Species studied: human, mouse

Enzymes shown to modify site in vitro:

Type	Enzyme
KINASE	Akt1 (human)

Upstream Regulation

Potential in vivo enzymes for site:

Type	Enzyme	Evidence	Notes
KINASE	Akt1 (mouse)	phospho-antibody, phospho-motif antibody, siRNA inhibition of enzyme, modification site within consensus motif, pharmacological activator of upstream enzyme

Treatments, proteins and their effect on site modification:

Treatments	Referenced Treatments	Manipulated Protein	Referenced Protein	Effect	Notes
insulin				increase

Downstream Regulation

Effect of modification (function): activation

Comments: nucleus morphology

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