Curated Information
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Curated Information Page
PubMed Id: 15722337 
This page summarizes selected information from the article referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Urschel S, et al. (2005) Phosphorylation of grb10 regulates its interaction with 14-3-3. J Biol Chem 280, 16987-93 15722337
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
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S473-p - Akt1 (mouse)
Orthologous residues
Akt1 (human): S473‑p, Akt1 (mouse): S473‑p, Akt1 (rat): S473‑p, Akt1 (fruit fly): S586‑p, Akt1 (cow): S473‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody
 Relevant cell lines - cell types - tissues:  293 (epithelial), BaF3 ('B lymphocyte, precursor'), COS (fibroblast), HeLa (cervical), U2OS (bone cell) [GR (human)]
 Cellular systems studied:  cell lines
 Species studied:  human, monkey, mouse
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
okadaic acid increase

S455-p - Grb10 (mouse)
Orthologous residues
Grb10 (human): S428‑p, Grb10 (mouse): S455‑p, Grb10 (rat): S433‑p
Characterization
 Methods used to characterize site in vivo [32P] bio-synthetic labeling, electrophoretic mobility shift, mutation of modification site, phospho-antibody
 Relevant cell lines - cell types - tissues:  293 (epithelial), BaF3 ('B lymphocyte, precursor'), COS (fibroblast), HeLa (cervical), U2OS (bone cell) [GR (human)]
 Cellular systems studied:  cell lines
 Species studied:  human, monkey, mouse
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE Akt1 (mouse)
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
okadaic acid increase
Downstream Regulation
 Effect of modification (function):  molecular association, regulation
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
14-3-3 eta (mouse) Induces yeast two-hybrid, co-immunoprecipitation, electrophoretic visualization


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