Curated Information
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Curated Information Page
PubMed Id: 15574412 
This page summarizes selected information from the article referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Information from this record has been curated, but not yet edited in PhosphoSitePlus® and may be incomplete.
Liberman Z, Eldar-Finkelman H (2005) Serine 332 phosphorylation of insulin receptor substrate-1 by glycogen synthase kinase-3 attenuates insulin signaling. J Biol Chem 280, 4422-8 15574412
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
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T308-p - Akt1 (rat)
Orthologous residues
Akt1 (human): T308‑p, Akt1 (mouse): T308‑p, Akt1 (rat): T308‑p, Akt1 (fruit fly): T423‑p, Akt1 (cow): T308‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  CHO (fibroblast) [InsR (human)]
 Cellular systems studied:  cell lines
 Species studied:  hamster
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
insulin increase

S473-p - Akt1 (rat)
Orthologous residues
Akt1 (human): S473‑p, Akt1 (mouse): S473‑p, Akt1 (rat): S473‑p, Akt1 (fruit fly): S586‑p, Akt1 (cow): S473‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  CHO (fibroblast) [InsR (human)]
 Cellular systems studied:  cell lines
 Species studied:  hamster
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
insulin increase

S332-p - IRS1 (rat)
Orthologous residues
IRS1 (human): S337‑p, IRS1 (mouse): S332‑p, IRS1 (rat): S332‑p
Characterization
 Methods used to characterize site in vivo mutation of modification site, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  293 (epithelial), CHO (fibroblast) [InsR (human)]
 Cellular systems studied:  cell lines
 Species studied:  hamster, human
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE GSK3B (human)
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE GSK3B (human) transfection of wild-type enzyme, pharmacological inhibitor of upstream enzyme, phospho-antibody, pharmacological activator of upstream enzyme
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
lithium decrease
insulin no change compared to control
Downstream Regulation
 Effect of modification (function):  phosphorylation
 Comments:  decreases tyrosine phosphorylation of IRS-1 and Akt1 phosphorylation upon insulin stimulation

S336-p - IRS1 (rat)
Orthologous residues
IRS1 (human): S341‑p, IRS1 (mouse): S336‑p, IRS1 (rat): S336‑p
Characterization
 Methods used to characterize site in vivo mutation of modification site
 Relevant cell lines - cell types - tissues:  293 (epithelial), CHO (fibroblast) [InsR (human)]
 Cellular systems studied:  cell lines
 Species studied:  hamster, human
Downstream Regulation
 Effect of modification (function):  phosphorylation
 Comments:  decreases tyrosine phosphorylation of IRS-1 and Akt1 phosphorylation upon insulin stimulation; primes S332 for phosphorylation by GSK3B


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