Curated Information
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Curated Information Page
PubMed Id: 14701740 
Ross SE, et al. (2004) Phosphorylation of C/EBPalpha inhibits granulopoiesis. Mol Cell Biol 24, 675-86 14701740
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
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S21-p - C/EBP-alpha (human)
Orthologous residues
C/EBP‑alpha (human): S21‑p, C/EBP‑alpha (mouse): S21‑p, C/EBP‑alpha (rat): S21‑p
Characterization
 Methods used to characterize site in vivo [32P] bio-synthetic labeling, mutation of modification site, peptide sequencing, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  293T (epithelial), 3T3 (fibroblast) [SHP-2 (mouse), homozygous knockout], adipose tissue, bone marrow, liver, lung
 Cellular systems studied:  cell lines, tissue
 Species studied:  human, mouse
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE ERK2 (human)
KINASE ERK1 (human)
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE ERK1 (human) pharmacological activator of upstream enzyme, pharmacological inhibitor of upstream enzyme, mutation in upstream enzyme recognition motif
KINASE ERK2 (human) pharmacological activator of upstream enzyme, pharmacological inhibitor of upstream enzyme, mutation in upstream enzyme recognition motif
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
PDGF increase
U0126 PD98059 inhibit treatment-induced increase
serum increase
U0126 serum inhibit treatment-induced increase
phorbol ester increase
U0126 phorbol ester inhibit treatment-induced increase
PD98059 phorbol ester inhibit treatment-induced increase
insulin increase
Downstream Regulation
 Effect of modification (function):  activity, inhibited, protein conformation


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