Curated Information
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Curated Information Page
PubMed Id: 15664191 
This page summarizes selected information from the article referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Dougherty MK, et al. (2005) Regulation of Raf-1 by direct feedback phosphorylation. Mol Cell 17, 215-24 15664191
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
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S29-p - c-Raf (human) ▲
Orthologous residues
c‑Raf (human): S29‑p, c‑Raf (mouse): S29‑p, c‑Raf (rat): S29‑p, c‑Raf (chicken): S29‑p
Characterization
 Methods used to characterize site in vivo:  electrophoretic mobility shift, mass spectrometry, mutation of modification site
 Relevant cell lines - cell types - tissues:  3T3 (fibroblast) [SHP-2 (mouse), homozygous knockout]
 Cellular systems studied:  cell lines
 Species studied:  mouse
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
PDGF increase
U0126 PDGF inhibit treatment-induced increase
okadaic acid PDGF augment treatment-induced increase
Downstream Regulation
 Effect of modification (function):  enzymatic activity, inhibited, intracellular localization, molecular association, regulation
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
HRas (human) Disrupts co-immunoprecipitation, in vitro

S43-p - c-Raf (human) ▲
Orthologous residues
c‑Raf (human): S43‑p, c‑Raf (mouse): S43‑p, c‑Raf (rat): S43‑p, c‑Raf (chicken): S43‑p
Characterization
 Methods used to characterize site in vivo:  electrophoretic mobility shift, mass spectrometry, mutation of modification site
 Relevant cell lines - cell types - tissues:  3T3 (fibroblast) [SHP-2 (mouse), homozygous knockout]
 Cellular systems studied:  cell lines
 Species studied:  mouse
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
PDGF increase
U0126 PDGF inhibit treatment-induced increase
okadaic acid PDGF augment treatment-induced increase
Downstream Regulation
 Effect of modification (function):  enzymatic activity, inhibited, intracellular localization, molecular association, regulation
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
HRas (human) Disrupts co-immunoprecipitation, in vitro

S259-p - c-Raf (human) ▲
Orthologous residues
c‑Raf (human): S259‑p, c‑Raf (mouse): S259‑p, c‑Raf (rat): S259‑p, c‑Raf (chicken): S259‑p
Characterization
 Methods used to characterize site in vivo:  electrophoretic mobility shift, mass spectrometry, mutation of modification site
 Relevant cell lines - cell types - tissues:  3T3 (fibroblast) [SHP-2 (mouse), homozygous knockout]
 Cellular systems studied:  cell lines
 Species studied:  mouse

S289-p - c-Raf (human) ▲
Orthologous residues
c‑Raf (human): S289‑p, c‑Raf (mouse): S289‑p, c‑Raf (rat): S289‑p, c‑Raf (chicken): S289‑p
Characterization
 Methods used to characterize site in vivo:  electrophoretic mobility shift, mass spectrometry, mutation of modification site
 Relevant cell lines - cell types - tissues:  3T3 (fibroblast) [SHP-2 (mouse), homozygous knockout]
 Cellular systems studied:  cell lines
 Species studied:  mouse
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
PDGF increase
U0126 PDGF inhibit treatment-induced increase
okadaic acid PDGF augment treatment-induced increase
Downstream Regulation
 Effect of modification (function):  enzymatic activity, inhibited, intracellular localization, molecular association, regulation
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
HRas (human) Disrupts co-immunoprecipitation, in vitro

S296-p - c-Raf (human) ▲
Orthologous residues
c‑Raf (human): S296‑p, c‑Raf (mouse): S296‑p, c‑Raf (rat): S296‑p, c‑Raf (chicken): S296‑p
Characterization
 Methods used to characterize site in vivo:  electrophoretic mobility shift, mass spectrometry, mutation of modification site
 Relevant cell lines - cell types - tissues:  3T3 (fibroblast) [SHP-2 (mouse), homozygous knockout]
 Cellular systems studied:  cell lines
 Species studied:  mouse
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
PDGF increase
U0126 PDGF inhibit treatment-induced increase
okadaic acid PDGF augment treatment-induced increase
Downstream Regulation
 Effect of modification (function):  enzymatic activity, inhibited, intracellular localization, molecular association, regulation
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
HRas (human) Disrupts co-immunoprecipitation, in vitro

S301-p - c-Raf (human) ▲
Orthologous residues
c‑Raf (human): S301‑p, c‑Raf (mouse): S301‑p, c‑Raf (rat): S301‑p, c‑Raf (chicken): S301‑p
Characterization
 Methods used to characterize site in vivo:  electrophoretic mobility shift, mass spectrometry, mutation of modification site
 Relevant cell lines - cell types - tissues:  3T3 (fibroblast) [SHP-2 (mouse), homozygous knockout]
 Cellular systems studied:  cell lines
 Species studied:  mouse
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
PDGF increase
U0126 PDGF inhibit treatment-induced increase
okadaic acid PDGF augment treatment-induced increase
Downstream Regulation
 Effect of modification (function):  enzymatic activity, inhibited, intracellular localization, molecular association, regulation
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
HRas (human) Disrupts co-immunoprecipitation, in vitro

S338-p - c-Raf (human) ▲
Orthologous residues
c‑Raf (human): S338‑p, c‑Raf (mouse): S338‑p, c‑Raf (rat): S338‑p, c‑Raf (chicken): S338‑p
Characterization
 Methods used to characterize site in vivo:  electrophoretic mobility shift, mass spectrometry, mutation of modification site, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  3T3 (fibroblast) [SHP-2 (mouse), homozygous knockout]
 Cellular systems studied:  cell lines
 Species studied:  mouse
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
PDGF increase

S621-p - c-Raf (human) ▲
Orthologous residues
c‑Raf (human): S621‑p, c‑Raf (mouse): S621‑p, c‑Raf (rat): S621‑p, c‑Raf (chicken): S621‑p
Characterization
 Methods used to characterize site in vivo:  electrophoretic mobility shift, mass spectrometry, mutation of modification site
 Relevant cell lines - cell types - tissues:  3T3 (fibroblast) [SHP-2 (mouse), homozygous knockout]
 Cellular systems studied:  cell lines
 Species studied:  mouse

S642-p - c-Raf (human) ▲
Orthologous residues
c‑Raf (human): S642‑p, c‑Raf (mouse): S642‑p, c‑Raf (rat): S642‑p, c‑Raf (chicken): T642‑p
Characterization
 Methods used to characterize site in vivo:  electrophoretic mobility shift, mass spectrometry, mutation of modification site, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  3T3 (fibroblast) [SHP-2 (mouse), homozygous knockout]
 Cellular systems studied:  cell lines
 Species studied:  mouse
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
PDGF increase
U0126 PDGF inhibit treatment-induced increase
okadaic acid PDGF augment treatment-induced increase
Downstream Regulation
 Effect of modification (function):  enzymatic activity, inhibited, intracellular localization, molecular association, regulation
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
HRas (human) Disrupts co-immunoprecipitation, in vitro


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