Curated Information
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Curated Information Page
PubMed Id: 9092573 
This page summarizes selected information from the article referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Fadden P, Haystead TA, Lawrence JC (1997) Identification of phosphorylation sites in the translational regulator, PHAS-I, that are controlled by insulin and rapamycin in rat adipocytes. J Biol Chem 272, 10240-7 9092573
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
Download Sites

T36-p - 4E-BP1 (rat) ▲
Orthologous residues
4E‑BP1 (human): T37‑p, 4E‑BP1 (mouse): T36‑p, 4E‑BP1 (rat): T36‑p, 4E‑BP1 (fruit fly): T37‑p
Characterization
 Methods used to characterize site in vivo:  [32P] bio-synthetic labeling, phosphopeptide mapping
 Relevant cell lines - cell types - tissues:  adipocyte-adipose tissue
 Cellular systems studied:  primary cells
 Species studied:  rat
 Enzymes shown to modify site in vitro: 
Type Enzyme
KINASE ERK2 (human)
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
rapamycin decrease
insulin increase
rapamycin insulin inhibit treatment-induced increase

T45-p - 4E-BP1 (rat) ▲
Orthologous residues
4E‑BP1 (human): T46‑p, 4E‑BP1 (mouse): T45‑p, 4E‑BP1 (rat): T45‑p, 4E‑BP1 (fruit fly): T46‑p
Characterization
 Methods used to characterize site in vivo:  [32P] bio-synthetic labeling, phosphopeptide mapping
 Relevant cell lines - cell types - tissues:  adipocyte-adipose tissue
 Cellular systems studied:  primary cells
 Species studied:  rat
 Enzymes shown to modify site in vitro: 
Type Enzyme
KINASE ERK2 (human)
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
rapamycin decrease
insulin increase
rapamycin insulin inhibit treatment-induced increase

S64-p - 4E-BP1 (rat) ▲
Orthologous residues
4E‑BP1 (human): S65‑p, 4E‑BP1 (mouse): S64‑p, 4E‑BP1 (rat): S64‑p, 4E‑BP1 (fruit fly): S65‑p
Characterization
 Methods used to characterize site in vivo:  [32P] bio-synthetic labeling, phosphopeptide mapping
 Relevant cell lines - cell types - tissues:  adipocyte-adipose tissue
 Cellular systems studied:  primary cells
 Species studied:  rat
 Enzymes shown to modify site in vitro: 
Type Enzyme
KINASE ERK2 (human)
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
rapamycin decrease
insulin increase
rapamycin insulin inhibit treatment-induced increase

T69-p - 4E-BP1 (rat) ▲
Orthologous residues
4E‑BP1 (human): T70‑p, 4E‑BP1 (mouse): T69‑p, 4E‑BP1 (rat): T69‑p, 4E‑BP1 (fruit fly): T70‑p
Characterization
 Methods used to characterize site in vivo:  [32P] bio-synthetic labeling, phosphopeptide mapping
 Relevant cell lines - cell types - tissues:  adipocyte-adipose tissue
 Cellular systems studied:  primary cells
 Species studied:  rat
 Enzymes shown to modify site in vitro: 
Type Enzyme
KINASE ERK2 (human)
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
insulin increase
rapamycin decrease
rapamycin insulin inhibit treatment-induced increase

S82-p - 4E-BP1 (rat) ▲
Orthologous residues
4E‑BP1 (human): S83‑p, 4E‑BP1 (mouse): S82‑p, 4E‑BP1 (rat): S82‑p, 4E‑BP1 (fruit fly): T83‑p
Characterization
 Methods used to characterize site in vivo:  [32P] bio-synthetic labeling, phosphopeptide mapping
 Relevant cell lines - cell types - tissues:  adipocyte-adipose tissue
 Cellular systems studied:  primary cells
 Species studied:  rat
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
rapamycin decrease
insulin increase
rapamycin insulin inhibit treatment-induced increase


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