Curated Information
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Curated Information Page
PubMed Id: 15642359 
Hekman M, et al. (2005) Novel C-Raf phosphorylation sites: serine 296 and 301 participate in Raf regulation. FEBS Lett 579, 464-8 15642359
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
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S259-p - RAF1 (human)
Orthologous residues
RAF1 (human): S259‑p, RAF1 (mouse): S259‑p, RAF1 (rat): S259‑p, RAF1 (chicken): S259‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  SF9
 Cellular systems studied:  cell lines
 Species studied:  insect

S296-p - RAF1 (human)
Orthologous residues
RAF1 (human): S296‑p, RAF1 (mouse): S296‑p, RAF1 (rat): S296‑p, RAF1 (chicken): S296‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry, mutation of modification site
 Relevant cell lines - cell types - tissues:  COS (fibroblast), SF9
 Cellular systems studied:  cell lines
 Species studied:  insect, monkey
Downstream Regulation
 Effect of modification (function):  enzymatic activity, inhibited

S301-p - RAF1 (human)
Orthologous residues
RAF1 (human): S301‑p, RAF1 (mouse): S301‑p, RAF1 (rat): S301‑p, RAF1 (chicken): S301‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry, mutation of modification site
 Relevant cell lines - cell types - tissues:  COS (fibroblast), SF9
 Cellular systems studied:  cell lines
 Species studied:  insect, monkey
Downstream Regulation
 Effect of modification (function):  enzymatic activity, inhibited

S621-p - RAF1 (human)
Orthologous residues
RAF1 (human): S621‑p, RAF1 (mouse): S621‑p, RAF1 (rat): S621‑p, RAF1 (chicken): S621‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  SF9
 Cellular systems studied:  cell lines
 Species studied:  insect


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