Curated Information
Javascript is not enabled on this browser. This site will not work properly without Javascript.
PhosphoSitePlus Homepage Cell Signaling Technology
PhosphoSitePlus
HomeAbout PhosphoSiteUsing PhosphoSiteCuration ProcessContact
NIH-logos NIGMS Logo NIAAA Logo NCI Logo NIH Logo
Curated Information Page
PubMed Id: 12167711 
This page summarizes selected information from the article referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Blattner C, Hay T, Meek DW, Lane DP (2002) Hypophosphorylation of Mdm2 augments p53 stability. Mol Cell Biol 22, 6170-82 12167711
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
Download Sites

S240-p - MDM2 (human)
Orthologous residues
MDM2 (human): S240‑p, MDM2 iso12 (human): , MDM2 (mouse): S238‑p
Characterization
 Methods used to characterize site in vivo [32P] bio-synthetic labeling, mutation of modification site, phospho-antibody, phosphopeptide mapping, western blotting
 Disease tissue studied:  lung cancer, non-small cell lung cancer
 Relevant cell lines - cell types - tissues:  COS (fibroblast), GM1604 (fibroblast), NCI-H1299 (pulmonary)
 Cellular systems studied:  cell lines
 Species studied:  human, monkey
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
ionizing radiation decrease
Downstream Regulation
 Effect of modification (function):  activity, induced
 Comments:  promotes p53 degradation

S242-p - MDM2 (human)
Orthologous residues
MDM2 (human): S242‑p, MDM2 iso12 (human): , MDM2 (mouse): S240‑p
Characterization
 Methods used to characterize site in vivo [32P] bio-synthetic labeling, mutation of modification site, phospho-antibody, phosphopeptide mapping, western blotting
 Disease tissue studied:  lung cancer, non-small cell lung cancer
 Relevant cell lines - cell types - tissues:  COS (fibroblast), GM1604 (fibroblast), NCI-H1299 (pulmonary)
 Cellular systems studied:  cell lines
 Species studied:  human, monkey
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
ionizing radiation decrease
Downstream Regulation
 Effect of modification (function):  activity, induced
 Comments:  promotes p53 degradation

S246-p - MDM2 (human)
Orthologous residues
MDM2 (human): S246‑p, MDM2 iso12 (human): , MDM2 (mouse): S244‑p
Characterization
 Methods used to characterize site in vivo [32P] bio-synthetic labeling, mutation of modification site, phospho-antibody, phosphopeptide mapping, western blotting
 Disease tissue studied:  lung cancer, non-small cell lung cancer
 Relevant cell lines - cell types - tissues:  COS (fibroblast), GM1604 (fibroblast), NCI-H1299 (pulmonary)
 Cellular systems studied:  cell lines
 Species studied:  human, monkey
Downstream Regulation
 Effect of modification (function):  activity, induced
 Comments:  promotes p53 degradation

S253-p - MDM2 (human)
Orthologous residues
MDM2 (human): S253‑p, MDM2 iso12 (human): , MDM2 (mouse): S251‑p
Characterization
 Methods used to characterize site in vivo [32P] bio-synthetic labeling, mutation of modification site, phospho-antibody, phosphopeptide mapping, western blotting
 Disease tissue studied:  lung cancer, non-small cell lung cancer
 Relevant cell lines - cell types - tissues:  COS (fibroblast), GM1604 (fibroblast), NCI-H1299 (pulmonary)
 Cellular systems studied:  cell lines
 Species studied:  human, monkey
Downstream Regulation
 Effect of modification (function):  activity, induced
 Comments:  promotes p53 degradation

S256-p - MDM2 (human)
Orthologous residues
MDM2 (human): S256‑p, MDM2 iso12 (human): , MDM2 (mouse): S254‑p
Characterization
 Methods used to characterize site in vivo [32P] bio-synthetic labeling, mutation of modification site, phospho-antibody, phosphopeptide mapping, western blotting
 Disease tissue studied:  lung cancer, non-small cell lung cancer
 Relevant cell lines - cell types - tissues:  COS (fibroblast), GM1604 (fibroblast), NCI-H1299 (pulmonary)
 Cellular systems studied:  cell lines
 Species studied:  human, monkey
Downstream Regulation
 Effect of modification (function):  activity, induced
 Comments:  promotes p53 degradation

S260-p - MDM2 (human)
Orthologous residues
MDM2 (human): S260‑p, MDM2 iso12 (human): , MDM2 (mouse): S258‑p
Characterization
 Methods used to characterize site in vivo [32P] bio-synthetic labeling, mutation of modification site, phospho-antibody, phosphopeptide mapping, western blotting
 Disease tissue studied:  lung cancer, non-small cell lung cancer
 Relevant cell lines - cell types - tissues:  COS (fibroblast), GM1604 (fibroblast), NCI-H1299 (pulmonary)
 Cellular systems studied:  cell lines
 Species studied:  human, monkey
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
ionizing radiation decrease
Downstream Regulation
 Effect of modification (function):  activity, induced
 Comments:  promotes p53 degradation

S262-p - MDM2 (human)
Orthologous residues
MDM2 (human): S262‑p, MDM2 iso12 (human): , MDM2 (mouse): S260‑p
Characterization
 Methods used to characterize site in vivo [32P] bio-synthetic labeling, mutation of modification site, phospho-antibody, phosphopeptide mapping, western blotting
 Disease tissue studied:  lung cancer, non-small cell lung cancer
 Relevant cell lines - cell types - tissues:  COS (fibroblast), GM1604 (fibroblast), NCI-H1299 (pulmonary)
 Cellular systems studied:  cell lines
 Species studied:  human, monkey
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
ionizing radiation decrease
Downstream Regulation
 Effect of modification (function):  activity, induced
 Comments:  promotes p53 degradation


Home  |  Curator Login With enhanced literature mining using Linguamatics I2E I2E Logo Produced by 3rd Millennium  |  Design by Digizyme
©2003-2013 Cell Signaling Technology, Inc.