Curated Information
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Curated Information Page
PubMed Id: 15584898 
Tsumura Y, et al. (2005) Sprouty-4 negatively regulates cell spreading by inhibiting the kinase activity of testicular protein kinase. Biochem J 387, 627-37 15584898
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Click on the protein name to open the protein page, and on the RSD number to open the site page.
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S3-p - Cofilin-1 (human)
Orthologous residues
Cofilin‑1 (human): S3‑p, Cofilin‑1 (mouse): S3‑p, Cofilin‑1 (rat): S3‑p, Cofilin‑1 (pig): S3‑p
 Methods used to characterize site in vivo [32P] bio-synthetic labeling, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  COS (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  monkey
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE TESK1 (human) transfection of wild-type enzyme
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
laminin-1 increase
laminin-1 SPRY4 (human) inhibit treatment-induced increase
laminin-1 TESK1 (human) augment treatment-induced increase
laminin-1 TESK1 (human), SPRY4 (human) inhibit treatment-induced increase

Y52-p - SPRY4 (human)
Orthologous residues
SPRY4 (human): Y52‑p, SPRY4 (mouse): Y53‑p, SPRY4 (rat): Y53‑p
 Methods used to characterize site in vivo mutation of modification site
 Relevant cell lines - cell types - tissues:  293T (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human

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