Curated Information
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Curated Information Page
PubMed Id: 7774578 
This page summarizes selected information from the article referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Wieser R, Wrana JL, Massagué J (1995) GS domain mutations that constitutively activate T beta R-I, the downstream signaling component in the TGF-beta receptor complex. EMBO J 14, 2199-208 7774578
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
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T185-p - TGFBR1 (human)
Orthologous residues
TGFBR1 (human): T185‑p, TGFBR1 (mouse): T185‑p
Characterization
 Methods used to characterize site in vivo [32P] bio-synthetic labeling, mutation of modification site, phosphopeptide mapping
 Relevant cell lines - cell types - tissues:  R-1B ('epithelial, lung')
 Cellular systems studied:  cell lines
 Species studied:  mink
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE TGFBR2 (human) co-immunoprecipitation, transfection of wild-type enzyme, transfection of inactive enzyme
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
TGF-beta increase

T186-p - TGFBR1 (human)
Orthologous residues
TGFBR1 (human): T186‑p, TGFBR1 (mouse): T186‑p
Characterization
 Methods used to characterize site in vivo [32P] bio-synthetic labeling, mutation of modification site, phosphopeptide mapping
 Relevant cell lines - cell types - tissues:  R-1B ('epithelial, lung')
 Cellular systems studied:  cell lines
 Species studied:  mink
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE TGFBR2 (human) co-immunoprecipitation, transfection of wild-type enzyme, transfection of inactive enzyme
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
TGF-beta increase

S187-p - TGFBR1 (human)
Orthologous residues
TGFBR1 (human): S187‑p, TGFBR1 (mouse): S187‑p
Characterization
 Methods used to characterize site in vivo [32P] bio-synthetic labeling, mutation of modification site, phosphopeptide mapping
 Relevant cell lines - cell types - tissues:  R-1B ('epithelial, lung')
 Cellular systems studied:  cell lines
 Species studied:  mink
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE TGFBR2 (human) co-immunoprecipitation, transfection of wild-type enzyme, transfection of inactive enzyme
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
TGF-beta increase

S189-p - TGFBR1 (human)
Orthologous residues
TGFBR1 (human): S189‑p, TGFBR1 (mouse): S189‑p
Characterization
 Methods used to characterize site in vivo [32P] bio-synthetic labeling, mutation of modification site, phosphopeptide mapping
 Relevant cell lines - cell types - tissues:  R-1B ('epithelial, lung')
 Cellular systems studied:  cell lines
 Species studied:  mink
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE TGFBR2 (human) co-immunoprecipitation, transfection of wild-type enzyme, transfection of inactive enzyme
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
TGF-beta increase

S191-p - TGFBR1 (human)
Orthologous residues
TGFBR1 (human): S191‑p, TGFBR1 (mouse): S191‑p
Characterization
 Methods used to characterize site in vivo [32P] bio-synthetic labeling, mutation of modification site, phosphopeptide mapping
 Relevant cell lines - cell types - tissues:  R-1B ('epithelial, lung')
 Cellular systems studied:  cell lines
 Species studied:  mink
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE TGFBR2 (human) co-immunoprecipitation, transfection of wild-type enzyme, transfection of inactive enzyme
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
TGF-beta increase

T200-p - TGFBR1 (human)
Orthologous residues
TGFBR1 (human): T200‑p, TGFBR1 (mouse): T200‑p
Characterization
 Methods used to characterize site in vivo [32P] bio-synthetic labeling, mutation of modification site, phosphopeptide mapping
 Relevant cell lines - cell types - tissues:  R-1B ('epithelial, lung')
 Cellular systems studied:  cell lines
 Species studied:  mink
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
TGF-beta increase
Downstream Regulation
 Effect of modification (function):  enzymatic activity, induced, phosphorylation
 Comments:  T200V mutation prevented ligand-induced phosphorylation at other residues

T204-p - TGFBR1 (human)
Orthologous residues
TGFBR1 (human): T204‑p, TGFBR1 (mouse): T204‑p
Characterization
 Methods used to characterize site in vivo [32P] bio-synthetic labeling, mutation of modification site, phosphopeptide mapping
 Relevant cell lines - cell types - tissues:  R-1B ('epithelial, lung')
 Cellular systems studied:  cell lines
 Species studied:  mink
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
TGF-beta increase
Downstream Regulation
 Effect of modification (function):  enzymatic activity, induced, phosphorylation


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