Curated Information
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PubMed Id: 15538382 
Essers MA, et al. (2004) FOXO transcription factor activation by oxidative stress mediated by the small GTPase Ral and JNK. EMBO J 23, 4802-12 15538382
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Click on the protein name to open the protein page, and on the RSD number to open the site page.
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T32-p - FOXO4 (human)
Orthologous residues
FOXO4 (human): T32‑p, FOXO4 (mouse): T32‑p, FOXO4 (rat): T32‑p
Characterization
 Methods used to characterize site in vivo [32P] bio-synthetic labeling, mutation of modification site, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  293T (epithelial), 3T3 (fibroblast) [SHP-2 (mouse), homozygous knockout], C2C12 (myoblast)
 Cellular systems studied:  cell lines
 Species studied:  human, mouse
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
insulin increase

S197-p - FOXO4 (human)
Orthologous residues
FOXO4 (human): S197‑p, FOXO4 (mouse): S197‑p, FOXO4 (rat): S197‑p
Characterization
 Methods used to characterize site in vivo [32P] bio-synthetic labeling, mutation of modification site, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  293T (epithelial), 3T3 (fibroblast) [SHP-2 (mouse), homozygous knockout], C2C12 (myoblast)
 Cellular systems studied:  cell lines
 Species studied:  human, mouse
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
insulin increase

T451-p - FOXO4 (human)
Orthologous residues
FOXO4 (human): T451‑p, FOXO4 (mouse): T452‑p, FOXO4 (rat): T452‑p
Characterization
 Methods used to characterize site in vivo [32P] bio-synthetic labeling, mutation of modification site, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  293T (epithelial), 3T3 (fibroblast) [SHP-2 (mouse), homozygous knockout], C2C12 (myoblast)
 Cellular systems studied:  cell lines
 Species studied:  human, mouse
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE JNK1 (human)
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE JNK1 (human) genetic knockout/knockin of upstream enzyme
KINASE JNK2 (human) genetic knockout/knockin of upstream enzyme
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
H2O2 increase
Downstream Regulation
 Effect of modification (function):  intracellular localization
 Effect of modification (process):  transcription, induced

T455-p - FOXO4 (human)
Orthologous residues
FOXO4 (human): T455‑p, FOXO4 (mouse): A456‑p, FOXO4 (rat): A456‑p
Characterization
 Methods used to characterize site in vivo [32P] bio-synthetic labeling, mutation of modification site, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  293T (epithelial), 3T3 (fibroblast) [SHP-2 (mouse), homozygous knockout], C2C12 (myoblast)
 Cellular systems studied:  cell lines
 Species studied:  human, mouse
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE JNK1 (human)
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE JNK1 (human) genetic knockout/knockin of upstream enzyme
KINASE JNK2 (human) genetic knockout/knockin of upstream enzyme
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
H2O2 increase
PD98059 H2O2 no effect upon treatment-induced increase
LY294002 H2O2 no effect upon treatment-induced increase
SB203580 H2O2 no effect upon treatment-induced increase
RALA (human) increase
Downstream Regulation
 Effect of modification (function):  intracellular localization
 Effect of modification (process):  transcription, induced


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