Curated Information
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Curated Information Page
PubMed Id: 18496528 
This page summarizes selected information from the article referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Stipanovich A, et al. (2008) A phosphatase cascade by which rewarding stimuli control nucleosomal response. Nature 453, 879-84 18496528
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
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S11-p - H3 (human)
Orthologous residues
H3 (human): S11‑p, H3 (mouse): S11‑p, H3 iso2 (mouse): S11‑p, H3 iso3 (mouse): S11‑p, H3 (rat): S11‑p, H3 iso3 (rat): S11‑p, H3 (pig): S11‑p, H3 (chicken): S11‑p, H3 (cow): S11‑p
Characterization
 Methods used to characterize site in vivo modification-specific antibody, mutation of modification site, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  'brain, nucleus accumbens', 'brain, striatum', 293 (epithelial)
 Cellular systems studied:  cell lines, primary cells, tissue
 Species studied:  human, mouse
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
cocaine increase
SKF81297 increase

K15-ac - H3 (human)
Orthologous residues
H3 (human): K15‑ac, H3 (mouse): K15‑ac, H3 iso2 (mouse): K15‑ac, H3 iso3 (mouse): K15‑ac, H3 (rat): K15‑ac, H3 iso3 (rat): K15‑ac, H3 (pig): K15‑ac, H3 (chicken): K15‑ac, H3 (cow): K15‑ac
Characterization
 Methods used to characterize site in vivo modification-specific antibody, mutation of modification site, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  'brain, nucleus accumbens', 'brain, striatum', 293 (epithelial)
 Cellular systems studied:  cell lines, primary cells, tissue
 Species studied:  human, mouse
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
SKF81297 no change compared to control

T34-p - DARPP-32 (mouse)
Orthologous residues
DARPP‑32 (human): T34‑p, DARPP‑32 (mouse): T34‑p, DARPP‑32 (rat): T34‑p, DARPP‑32 (cow): T34‑p
Characterization
 Methods used to characterize site in vivo modification-specific antibody, mutation of modification site, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  'brain, nucleus accumbens', 'brain, striatum', 293 (epithelial)
 Cellular systems studied:  cell lines, primary cells, tissue
 Species studied:  human, mouse
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
amphetamine increase
SKF81297 increase
forskolin increase
okadaic acid forskolin augment treatment-induced increase
okadaic acid increase
Downstream Regulation
 Effect of modification (function):  phosphorylation
 Comments:  histone H3 phosphorylation

S97-p - DARPP-32 (mouse)
Orthologous residues
DARPP‑32 (human): S102‑p, DARPP‑32 (mouse): S97‑p, DARPP‑32 (rat): S102‑p, DARPP‑32 (cow): S102‑p
Characterization
 Methods used to characterize site in vivo modification-specific antibody, mutation of modification site, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  'brain, nucleus accumbens', 'brain, striatum', 293 (epithelial)
 Cellular systems studied:  cell lines, primary cells, tissue
 Species studied:  human, mouse
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
PHOSPHATASE PPP2CA (mouse) pharmacological inhibitor of upstream enzyme, activation of upstream enzyme, phospho-antibody
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
SKF81297 decrease
forskolin decrease
okadaic acid forskolin inhibit treatment-induced decrease
okadaic acid increase
Downstream Regulation
 Effect of modification (function):  intracellular localization, phosphorylation
 Comments:  histone H3 phosphorylation


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