Curated Information
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PubMed Id: 18387945 
This page summarizes selected information from the article referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Fang WB, et al. (2008) Identification and functional analysis of phosphorylated tyrosine residues within EphA2 receptor tyrosine kinase. J Biol Chem 283, 16017-26 18387945
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
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Y588-p - EphA2 (human) ▲
Orthologous residues
EphA2 (human): Y588‑p, EphA2 (mouse): Y589‑p, EphA2 (rat): Y589‑p
Characterization
 Methods used to characterize site in vivo:  mutation of modification site, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  microvessel endothelial-lung
 Cellular systems studied:  primary cultured cells
 Species studied:  mouse
 Enzymes shown to modify site in vitro: 
Type Enzyme
KINASE EphA2 (human)
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
ephrin A1 increase
Downstream Regulation
 Effect of modification (function):  enzymatic activity, induced, molecular association, regulation
 Effect of modification (process):  cell differentiation, altered, cell motility, altered
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
VAV3 (human) Induces co-immunoprecipitation
VAV2 (human) Induces co-immunoprecipitation

Y594-p - EphA2 (human) ▲
Orthologous residues
EphA2 (human): Y594‑p, EphA2 (mouse): Y595‑p, EphA2 (rat): Y595‑p
Characterization
 Methods used to characterize site in vivo:  mass spectrometry, mutation of modification site, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  COS (fibroblast), microvessel endothelial-lung
 Cellular systems studied:  cell lines, primary cultured cells
 Species studied:  human, mouse
 Enzymes shown to modify site in vitro: 
Type Enzyme
KINASE EphA2 (human)
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
ephrin A1 increase
Downstream Regulation
 Effect of modification (function):  enzymatic activity, induced, molecular association, regulation
 Effect of modification (process):  cell differentiation, altered, cell motility, altered
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
VAV3 (human) Induces co-immunoprecipitation
VAV2 (human) Induces co-immunoprecipitation

Y735-p - EphA2 (human) ▲
Orthologous residues
EphA2 (human): Y735‑p, EphA2 (mouse): Y736‑p, EphA2 (rat): Y736‑p
Characterization
 Methods used to characterize site in vivo:  mass spectrometry, mutation of modification site, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  COS (fibroblast), microvessel endothelial-lung
 Cellular systems studied:  cell lines, primary cultured cells
 Species studied:  human, mouse
 Enzymes shown to modify site in vitro: 
Type Enzyme
KINASE EphA2 (human)
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
ephrin A1 increase
Downstream Regulation
 Effect of modification (function):  molecular association, regulation
 Effect of modification (process):  cell differentiation, altered, cell motility, altered
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
PIK3R1 (human) Induces co-immunoprecipitation

Y772-p - EphA2 (human) ▲
Orthologous residues
EphA2 (human): Y772‑p, EphA2 (mouse): Y773‑p, EphA2 (rat): Y773‑p
Characterization
 Methods used to characterize site in vivo:  mass spectrometry, mutation of modification site, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  COS (fibroblast), microvessel endothelial-lung
 Cellular systems studied:  cell lines, primary cultured cells
 Species studied:  human, mouse
 Enzymes shown to modify site in vitro: 
Type Enzyme
KINASE EphA2 (human)
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
ephrin A1 increase

Y930-p - EphA2 (human) ▲
Orthologous residues
EphA2 (human): Y930‑p, EphA2 (mouse): Y931‑p, EphA2 (rat): Y931‑p
Characterization
 Methods used to characterize site in vivo:  mutation of modification site, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  microvessel endothelial-lung
 Cellular systems studied:  primary cultured cells
 Species studied:  mouse
Downstream Regulation
 Effect of modification (function):  enzymatic activity, induced, molecular association, regulation
 Effect of modification (process):  cell differentiation, altered
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
VAV3 (human) Induces co-immunoprecipitation
PIK3R1 (human) Induces co-immunoprecipitation


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