Curated Information
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Curated Information Page
PubMed Id: 15461589 
This page summarizes selected information from the article referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Information from this record has been curated, but not yet edited in PhosphoSitePlus® and may be incomplete.
Murray JT, et al. (2004) Exploitation of KESTREL to identify NDRG family members as physiological substrates for SGK1 and GSK3. Biochem J 384, 477-88 15461589
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
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S473-p - Akt1 (human)
Orthologous residues
Akt1 (human): S473‑p, Akt1 (mouse): S473‑p, Akt1 (rat): S473‑p, Akt1 (fruit fly): S586‑p, Akt1 (cow): S473‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  'muscle, skeletal' [SGK1 (human), homozygous knockout], HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  mouse
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
serum increase
siRNA serum SGK1 (human) no effect upon treatment-induced increase
IGF-1 increase
siRNA IGF-1 SGK1 (human) no effect upon treatment-induced increase

T328-p - NDRG1 (human)
Orthologous residues
NDRG1 (human): T328‑p, NDRG1 (mouse): T328‑p, NDRG1 (rat): T328‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  'muscle, skeletal' [SGK1 (human), homozygous knockout], HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  mouse
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE SGK1 (human)

S330-p - NDRG1 (human)
Orthologous residues
NDRG1 (human): S330‑p, NDRG1 (mouse): S330‑p, NDRG1 (rat): S330‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  'muscle, skeletal' [SGK1 (human), homozygous knockout], HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  mouse
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE SGK1 (human)

S342-p - NDRG1 (human)
Orthologous residues
NDRG1 (human): S342‑p, NDRG1 (mouse): S342‑p, NDRG1 (rat): S342‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  'muscle, skeletal' [SGK1 (human), homozygous knockout], HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  mouse
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE GSK3A (human)
 Comments:  SGK primes GSK3A phosphorylation of NDRG1.

T346-p - NDRG1 (human)
Orthologous residues
NDRG1 (human): T346‑p, NDRG1 (mouse): T346‑p, NDRG1 (rat): T346‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  'muscle, skeletal' [SGK1 (human), homozygous knockout], HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  mouse
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE SGK1 (human)
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE SGK1 (human) genetic knockout/knockin of upstream enzyme
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
IGF-1 increase
siRNA IGF-1 SGK1 (human) inhibit treatment-induced increase
serum increase
siRNA serum SGK1 (human) inhibit treatment-induced increase
LY294002 decrease
CT-99021 no change compared to control
serum increase
LY294002 serum inhibit treatment-induced increase
CT-99021 no effect upon treatment-induced increase

S352-p - NDRG1 (human)
Orthologous residues
NDRG1 (human): S352‑p, NDRG1 (mouse): S352‑p, NDRG1 (rat): S352‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  'muscle, skeletal' [SGK1 (human), homozygous knockout], HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  mouse
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE GSK3A (human)
 Comments:  SGK primes GSK3A phosphorylation of NDRG1.

T356-p - NDRG1 (human)
Orthologous residues
NDRG1 (human): T356‑p, NDRG1 (mouse): T356‑p, NDRG1 (rat): T356‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  'muscle, skeletal' [SGK1 (human), homozygous knockout], HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  mouse
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE SGK1 (human)
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE SGK1 (human) genetic knockout/knockin of upstream enzyme
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
IGF-1 increase
siRNA IGF-1 SGK1 (human) inhibit treatment-induced increase
serum increase
siRNA serum SGK1 (human) inhibit treatment-induced increase
LY294002 decrease
CT-99021 no change compared to control
serum increase
LY294002 serum inhibit treatment-induced increase
CT-99021 no effect upon treatment-induced increase

S362-p - NDRG1 (human)
Orthologous residues
NDRG1 (human): S362‑p, NDRG1 (mouse): S362‑p, NDRG1 (rat): S362‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  'muscle, skeletal' [SGK1 (human), homozygous knockout], HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  mouse
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE GSK3A (human)
 Comments:  SGK primes GSK3A phosphorylation of NDRG1.

T366-p - NDRG1 (human)
Orthologous residues
NDRG1 (human): T366‑p, NDRG1 (mouse): T366‑p, NDRG1 (rat): T366‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  'muscle, skeletal' [SGK1 (human), homozygous knockout], HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  mouse
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE SGK1 (human)
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE SGK1 (human) genetic knockout/knockin of upstream enzyme
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
IGF-1 increase
siRNA IGF-1 SGK1 (human) inhibit treatment-induced increase
serum increase
siRNA serum SGK1 (human) inhibit treatment-induced increase
LY294002 decrease
CT-99021 no change compared to control
serum increase
LY294002 serum inhibit treatment-induced increase
CT-99021 no effect upon treatment-induced increase

T330-p - NDRG2 (human)
Orthologous residues
NDRG2 (human): T330‑p, NDRG2 (mouse): T330‑p, NDRG2 (rat): T330‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  'muscle, skeletal' [SGK1 (human), homozygous knockout], HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  mouse
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE SGK1 (human)
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE SGK1 (human) genetic knockout/knockin of upstream enzyme

S332-p - NDRG2 (human)
Orthologous residues
NDRG2 (human): S332‑p, NDRG2 (mouse): S332‑p, NDRG2 (rat): S332‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  'muscle, skeletal' [SGK1 (human), homozygous knockout], HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  mouse
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE p90RSK (human)
KINASE Akt1 (human)
KINASE p70S6K (human)
KINASE SGK1 (human)
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE SGK1 (human) genetic knockout/knockin of upstream enzyme

S346-p - NDRG2 (human)
Orthologous residues
NDRG2 (human): S346‑p, NDRG2 (mouse): S346‑p, NDRG2 (rat): S346‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  'muscle, skeletal' [SGK1 (human), homozygous knockout], HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  mouse
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE SGK1 (human) genetic knockout/knockin of upstream enzyme

T348-p - NDRG2 (human)
Orthologous residues
NDRG2 (human): T348‑p, NDRG2 (mouse): T348‑p, NDRG2 (rat): T348‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  'muscle, skeletal' [SGK1 (human), homozygous knockout], HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  mouse
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE SGK1 (human)
KINASE Akt1 (human)
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE SGK1 (human) genetic knockout/knockin of upstream enzyme

S350-p - NDRG2 (human)
Orthologous residues
NDRG2 (human): S350‑p, NDRG2 (mouse): S350‑p, NDRG2 (rat): S350‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  'muscle, skeletal' [SGK1 (human), homozygous knockout], HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  mouse
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE p70S6K (human)
KINASE p90RSK (human)

S422-p - SGK1 (human)
Orthologous residues
SGK1 (human): S422‑p, SGK1 iso3 (human): S436‑p, SGK1 (mouse): S422‑p, SGK1 (rat): S421‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  'muscle, skeletal' [SGK1 (human), homozygous knockout], HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  mouse
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
IGF-1 increase
siRNA IGF-1 SGK1 (human) inhibit treatment-induced increase
serum increase
siRNA serum SGK1 (human) inhibit treatment-induced increase


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