Curated Information
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Curated Information Page
PubMed Id: 18167341 
This page summarizes selected information from the article referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Lee J, et al. (2008) Epigenetic-mediated dysfunction of the bone morphogenetic protein pathway inhibits differentiation of glioblastoma-initiating cells. Cancer Cell 13, 69-80 18167341
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
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S463-p - Smad1 (mouse)
Orthologous residues
Smad1 (human): S463‑p, Smad1 (mouse): S463‑p, Smad1 (rat): S466‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Disease tissue studied:  brain cancer, glioblastoma, glioma
 Relevant cell lines - cell types - tissues:  glial, stem, U251 (glial)
 Cellular systems studied:  primary cells, primary cultured cells
 Species studied:  human, mouse
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
BMP2 increase
CNTF no change compared to control
LIF no change compared to control

S465-p - Smad1 (mouse)
Orthologous residues
Smad1 (human): S465‑p, Smad1 (mouse): S465‑p, Smad1 (rat): S468‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Disease tissue studied:  brain cancer, glioblastoma, glioma
 Relevant cell lines - cell types - tissues:  glial, stem, U251 (glial)
 Cellular systems studied:  primary cells, primary cultured cells
 Species studied:  human, mouse
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
BMP2 increase
CNTF no change compared to control
LIF no change compared to control

S463-p - Smad5 (mouse)
Orthologous residues
Smad5 (human): S463‑p, Smad5 (mouse): S463‑p, Smad5 (rat): S463‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Disease tissue studied:  brain cancer, glioblastoma, glioma
 Relevant cell lines - cell types - tissues:  glial, stem, U251 (glial)
 Cellular systems studied:  primary cells, primary cultured cells
 Species studied:  human, mouse
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
BMP2 increase
CNTF no change compared to control
LIF no change compared to control

S465-p - Smad5 (mouse)
Orthologous residues
Smad5 (human): S465‑p, Smad5 (mouse): S465‑p, Smad5 (rat): S465‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Disease tissue studied:  brain cancer, glioblastoma, glioma
 Relevant cell lines - cell types - tissues:  glial, stem, U251 (glial)
 Cellular systems studied:  primary cells, primary cultured cells
 Species studied:  human, mouse
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
BMP2 increase
CNTF no change compared to control
LIF no change compared to control

S428-p - Smad8 (mouse)
Orthologous residues
Smad8 (human): S465‑p, Smad8 (mouse): S428‑p, Smad8 (rat): S432‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Disease tissue studied:  brain cancer, glioblastoma, glioma
 Relevant cell lines - cell types - tissues:  glial, stem, U251 (glial)
 Cellular systems studied:  primary cells, primary cultured cells
 Species studied:  human, mouse
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
BMP2 increase
CNTF no change compared to control
LIF no change compared to control

S430-p - Smad8 (mouse)
Orthologous residues
Smad8 (human): S467‑p, Smad8 (mouse): S430‑p, Smad8 (rat): S434‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Disease tissue studied:  brain cancer, glioblastoma, glioma
 Relevant cell lines - cell types - tissues:  glial, stem, U251 (glial)
 Cellular systems studied:  primary cells, primary cultured cells
 Species studied:  human, mouse
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
BMP2 increase
CNTF no change compared to control
LIF no change compared to control

Y705-p - STAT3 (mouse)
Orthologous residues
STAT3 (human): Y705‑p, STAT3 iso2 (human): Y704‑p, STAT3 (mouse): Y705‑p, STAT3 iso2 (mouse): Y705‑p, STAT3 iso3 (mouse): Y704‑p, STAT3 (rat): Y705‑p, STAT3 (cow): Y705‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Disease tissue studied:  brain cancer, glioblastoma, glioma
 Relevant cell lines - cell types - tissues:  glial, stem, U251 (glial)
 Cellular systems studied:  primary cells, primary cultured cells
 Species studied:  human, mouse
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
BMP2 no change compared to control
CNTF increase
LIF increase


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