Curated Information
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PubMed Id: 15486085 
Harada H, Quearry B, Ruiz-Vela A, Korsmeyer SJ (2004) Survival factor-induced extracellular signal-regulated kinase phosphorylates BIM, inhibiting its association with BAX and proapoptotic activity. Proc Natl Acad Sci U S A 101, 15313-7 15486085
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
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S55-p - BIM (mouse)
Orthologous residues
BIM (human): S59‑p, BIM iso2 (human): , BIM iso3 (human): , BIM (mouse): S55‑p, BIM (rat): S55‑p
Characterization
 Methods used to characterize site in vivo mutation of modification site, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  FL5.12 (lymphoid) [BIM (mouse)]
 Cellular systems studied:  cell lines
 Species studied:  mouse
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE ERK2 (mouse) pharmacological inhibitor of upstream enzyme
KINASE ERK1 (mouse) pharmacological inhibitor of upstream enzyme
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
IL-3 increase
U0126 IL-3 inhibit treatment-induced increase
PD98059 IL-3 inhibit treatment-induced increase
LY294002 IL-3 no effect upon treatment-induced increase
SP600125 IL-3 no effect upon treatment-induced increase
SB203580 IL-3 no effect upon treatment-induced increase
Downstream Regulation
 Effect of modification (function):  molecular association, regulation
 Effect of modification (process):  apoptosis, altered
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
BAX (mouse) Disrupts apoptosis, altered co-immunoprecipitation

S65-p - BIM (mouse)
Orthologous residues
BIM (human): S69‑p, BIM iso2 (human): , BIM iso3 (human): , BIM (mouse): S65‑p, BIM (rat): S65‑p
Characterization
 Methods used to characterize site in vivo mutation of modification site, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  FL5.12 (lymphoid) [BIM (mouse)]
 Cellular systems studied:  cell lines
 Species studied:  mouse
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE ERK1 (mouse) pharmacological inhibitor of upstream enzyme
KINASE ERK2 (mouse) pharmacological inhibitor of upstream enzyme
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
IL-3 increase
U0126 IL-3 inhibit treatment-induced increase
PD98059 IL-3 inhibit treatment-induced increase
LY294002 IL-3 no effect upon treatment-induced increase
SP600125 IL-3 no effect upon treatment-induced increase
SB203580 IL-3 no effect upon treatment-induced increase
Downstream Regulation
 Effect of modification (function):  molecular association, regulation
 Effect of modification (process):  apoptosis, altered
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
BAX (mouse) Disrupts apoptosis, altered co-immunoprecipitation

S100-p - BIM (mouse)
Orthologous residues
BIM (human): S104‑p, BIM iso2 (human): S44‑p, BIM iso3 (human): , BIM (mouse): S100‑p, BIM (rat): S100‑p
Characterization
 Methods used to characterize site in vivo mutation of modification site, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  FL5.12 (lymphoid) [BIM (mouse)]
 Cellular systems studied:  cell lines
 Species studied:  mouse
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE ERK1 (mouse) pharmacological inhibitor of upstream enzyme
KINASE ERK2 (mouse) pharmacological inhibitor of upstream enzyme
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
IL-3 increase
U0126 IL-3 inhibit treatment-induced increase
PD98059 IL-3 inhibit treatment-induced increase
LY294002 IL-3 no effect upon treatment-induced increase
SP600125 IL-3 no effect upon treatment-induced increase
SB203580 IL-3 no effect upon treatment-induced increase
Downstream Regulation
 Effect of modification (function):  molecular association, regulation
 Effect of modification (process):  apoptosis, altered
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
BAX (mouse) Disrupts apoptosis, altered co-immunoprecipitation


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