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Curated Information Page
PubMed Id: 18347057 
Bayascas JR, et al. (2008) Mutation of the PDK1 PH domain inhibits protein kinase B/Akt, leading to small size and insulin resistance. Mol Cell Biol 28, 3258-72 18347057
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
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T308-p - Akt1 (mouse)
Orthologous residues
Akt1 (human): T308‑p, Akt1 (mouse): T308‑p, Akt1 (rat): T308‑p, Akt1 (fruit fly): T423‑p, Akt1 (cow): T308‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  'muscle, skeletal', 'stem, embryonic', adipose tissue, heart, liver
 Cellular systems studied:  primary cultured cells, tissue
 Species studied:  mouse
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
insulin increase
insulin PDK1 (mouse) inhibit treatment-induced increase dominant negative (PH domain mutant)

S473-p - Akt1 (mouse)
Orthologous residues
Akt1 (human): S473‑p, Akt1 (mouse): S473‑p, Akt1 (rat): S473‑p, Akt1 (fruit fly): S586‑p, Akt1 (cow): S473‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  'muscle, skeletal', 'stem, embryonic', adipose tissue, heart, liver
 Cellular systems studied:  primary cultured cells, tissue
 Species studied:  mouse
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
insulin increase
insulin PDK1 (mouse) no effect upon treatment-induced increase dominant negative (PH domain mutant)

T203-p - ERK1 (mouse)
Orthologous residues
ERK1 (human): T202‑p, ERK1 (mouse): T203‑p, ERK1 (rat): T203‑p, ERK1 (hamster): T192‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  'muscle, skeletal', 'stem, embryonic', adipose tissue, heart, liver
 Cellular systems studied:  primary cultured cells, tissue
 Species studied:  mouse
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
insulin increase
insulin PDK1 (mouse) no effect upon treatment-induced increase dominant negative (PH domain mutant)

Y205-p - ERK1 (mouse)
Orthologous residues
ERK1 (human): Y204‑p, ERK1 (mouse): Y205‑p, ERK1 (rat): Y205‑p, ERK1 (hamster): Y194‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  'muscle, skeletal', 'stem, embryonic', adipose tissue, heart, liver
 Cellular systems studied:  primary cultured cells, tissue
 Species studied:  mouse
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
insulin increase
insulin PDK1 (mouse) no effect upon treatment-induced increase dominant negative (PH domain mutant)

T183-p - ERK2 (mouse)
Orthologous residues
ERK2 (human): T185‑p, ERK2 (mouse): T183‑p, ERK2 (rat): T183‑p, ERK2 (chicken): T193‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  'muscle, skeletal', 'stem, embryonic', adipose tissue, heart, liver
 Cellular systems studied:  primary cultured cells, tissue
 Species studied:  mouse
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
insulin increase
insulin PDK1 (mouse) no effect upon treatment-induced increase dominant negative (PH domain mutant)

Y185-p - ERK2 (mouse)
Orthologous residues
ERK2 (human): Y187‑p, ERK2 (mouse): Y185‑p, ERK2 (rat): Y185‑p, ERK2 (chicken): Y195‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  'muscle, skeletal', 'stem, embryonic', adipose tissue, heart, liver
 Cellular systems studied:  primary cultured cells, tissue
 Species studied:  mouse
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
insulin increase
insulin PDK1 (mouse) no effect upon treatment-induced increase dominant negative (PH domain mutant)

S253-p - FOXO1A (mouse)
Orthologous residues
FOXO1A (human): S256‑p, FOXO1A (mouse): S253‑p, FOXO1A (rat): S250‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  'muscle, skeletal', 'stem, embryonic', adipose tissue, heart, liver
 Cellular systems studied:  primary cultured cells, tissue
 Species studied:  mouse
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
insulin increase
insulin PDK1 (mouse) inhibit treatment-induced increase dominant negative (PH domain mutant)

S21-p - GSK3A (mouse)
Orthologous residues
GSK3A (human): S21‑p, GSK3A (mouse): S21‑p, GSK3A (rat): S21‑p, GSK3A (cow): S21‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  'muscle, skeletal', 'stem, embryonic', adipose tissue, heart, liver
 Cellular systems studied:  primary cultured cells, tissue
 Species studied:  mouse
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
insulin increase
insulin PDK1 (mouse) no effect upon treatment-induced increase dominant negative (PH domain mutant)

S9-p - GSK3B (mouse)
Orthologous residues
GSK3B (human): S9‑p, GSK3B iso2 (human): S9‑p, GSK3B (mouse): S9‑p, GSK3B (rat): S9‑p, GSK3B (rabbit): S3‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  'muscle, skeletal', 'stem, embryonic', adipose tissue, heart, liver
 Cellular systems studied:  primary cultured cells, tissue
 Species studied:  mouse
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
insulin increase
insulin PDK1 (mouse) no effect upon treatment-induced increase dominant negative (PH domain mutant)

Y1179-p - InsR (mouse)
Orthologous residues
InsR (human): Y1189‑p, InsR iso2 (human): Y1177‑p, InsR (mouse): Y1179‑p, InsR (rat): Y1190‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  'muscle, skeletal', 'stem, embryonic', adipose tissue, heart, liver
 Cellular systems studied:  primary cultured cells, tissue
 Species studied:  mouse
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
insulin increase
insulin PDK1 (mouse) no effect upon treatment-induced increase dominant negative (PH domain mutant)

Y1180-p - InsR (mouse)
Orthologous residues
InsR (human): Y1190‑p, InsR iso2 (human): Y1178‑p, InsR (mouse): Y1180‑p, InsR (rat): Y1191‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  'muscle, skeletal', 'stem, embryonic', adipose tissue, heart, liver
 Cellular systems studied:  primary cultured cells, tissue
 Species studied:  mouse
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
insulin increase
insulin PDK1 (mouse) no effect upon treatment-induced increase dominant negative (PH domain mutant)

T346-p - NDRG1 (mouse)
Orthologous residues
NDRG1 (human): T346‑p, NDRG1 (mouse): T346‑p, NDRG1 (rat): T346‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  'muscle, skeletal', 'stem, embryonic', adipose tissue, heart, liver
 Cellular systems studied:  primary cultured cells, tissue
 Species studied:  mouse
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
insulin increase
insulin PDK1 (mouse) no effect upon treatment-induced increase dominant negative (PH domain mutant)

T356-p - NDRG1 (mouse)
Orthologous residues
NDRG1 (human): T356‑p, NDRG1 (mouse): T356‑p, NDRG1 (rat): T356‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  'muscle, skeletal', 'stem, embryonic', adipose tissue, heart, liver
 Cellular systems studied:  primary cultured cells, tissue
 Species studied:  mouse
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
insulin increase
insulin PDK1 (mouse) no effect upon treatment-induced increase dominant negative (PH domain mutant)

T366-p - NDRG1 (mouse)
Orthologous residues
NDRG1 (human): T366‑p, NDRG1 (mouse): T366‑p, NDRG1 (rat): T366‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  'muscle, skeletal', 'stem, embryonic', adipose tissue, heart, liver
 Cellular systems studied:  primary cultured cells, tissue
 Species studied:  mouse
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
insulin increase
insulin PDK1 (mouse) no effect upon treatment-induced increase dominant negative (PH domain mutant)

T252-p - p70S6K (mouse)
Orthologous residues
p70S6K (human): T252‑p, p70S6K iso2 (human): T229‑p, p70S6K (mouse): T252‑p, p70S6K (rat): T252‑p, p70S6K iso2 (rat): T229‑p, p70S6K (fruit fly): T238‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  'muscle, skeletal', 'stem, embryonic', adipose tissue, heart, liver
 Cellular systems studied:  primary cultured cells, tissue
 Species studied:  mouse
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
insulin increase
insulin PDK1 (mouse) inhibit treatment-induced increase dominant negative (PH domain mutant)
meal feeding increase
meal feeding PDK1 (mouse) no effect upon treatment-induced increase dominant negative (PH domain mutant)

T412-p - p70S6K (mouse)
Orthologous residues
p70S6K (human): T412‑p, p70S6K iso2 (human): T389‑p, p70S6K (mouse): T412‑p, p70S6K (rat): T412‑p, p70S6K iso2 (rat): T389‑p, p70S6K (fruit fly): T398‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  'muscle, skeletal', 'stem, embryonic', adipose tissue, heart, liver
 Cellular systems studied:  primary cultured cells, tissue
 Species studied:  mouse
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
insulin increase
insulin PDK1 (mouse) inhibit treatment-induced increase dominant negative (PH domain mutant)
meal feeding increase
meal feeding PDK1 (mouse) no effect upon treatment-induced increase dominant negative (PH domain mutant)

T247-p - PRAS40 (mouse)
Orthologous residues
PRAS40 (human): T246‑p, PRAS40 iso3 (human): T266‑p, PRAS40 (mouse): T247‑p, PRAS40 (rat): T247‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  'muscle, skeletal', 'stem, embryonic', adipose tissue, heart, liver
 Cellular systems studied:  primary cultured cells, tissue
 Species studied:  mouse
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
insulin increase
insulin PDK1 (mouse) inhibit treatment-induced increase dominant negative (PH domain mutant)

S235-p - S6 (mouse)
Orthologous residues
S6 (human): S235‑p, S6 (mouse): S235‑p, S6 (rat): S235‑p, S6 (fruit fly): A234‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  'muscle, skeletal', 'stem, embryonic', adipose tissue, heart, liver
 Cellular systems studied:  primary cultured cells, tissue
 Species studied:  mouse
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
insulin increase
insulin PDK1 (mouse) inhibit treatment-induced increase dominant negative (PH domain mutant)
meal feeding increase
meal feeding PDK1 (mouse) no effect upon treatment-induced increase dominant negative (PH domain mutant)

T1465-p - TSC2 (mouse)
Orthologous residues
TSC2 (human): T1462‑p, TSC2 iso3 (human): T1418‑p, TSC2 iso4 (human): T1439‑p, TSC2 (mouse): T1465‑p, TSC2 iso6 (mouse): T1443‑p, TSC2 (rat): T1466‑p, TSC2 iso2 (rat): T1423‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  'muscle, skeletal', 'stem, embryonic', adipose tissue, heart, liver
 Cellular systems studied:  primary cultured cells, tissue
 Species studied:  mouse
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
insulin increase
insulin PDK1 (mouse) inhibit treatment-induced increase dominant negative (PH domain mutant)


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