Curated Information
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Curated Information Page
PubMed Id: 15342649 
This page summarizes selected information from the article referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Wang W, et al. (2004) AMP-activated protein kinase-regulated phosphorylation and acetylation of importin alpha1: involvement in the nuclear import of RNA-binding protein HuR. J Biol Chem 279, 48376-88 15342649
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
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K22-a - KPNA2 (human) ▲
Orthologous residues
KPNA2 (human): K22‑a, KPNA2 (mouse): K22‑a
Characterization
 Methods used to characterize site in vivo:  [32P] bio-synthetic labeling, immunoprecipitation, mutation of modification site
 Disease tissue studied:  colorectal cancer, colorectal carcinoma
 Relevant cell lines - cell types - tissues:  293 (epithelial) [KPNA2 (human)], RKO (intestinal)
 Cellular systems studied:  cell lines
 Enzymes shown to modify site in vitro: 
Type Enzyme
ACETYLTRANSFERASE p300 (human)
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
ACETYLTRANSFERASE p300 (human) transfection of wild-type enzyme
Downstream Regulation
 Effect of modification (function):  activation, intracellular localization, molecular association, regulation
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
HuR (human) Induces intracellular localization co-immunoprecipitation
 Comments:  promotes the nuclear localization of HuR

S105-p - KPNA2 (human) ▲
Orthologous residues
KPNA2 (human): S105‑p, KPNA2 (mouse): S105‑p
Characterization
 Methods used to characterize site in vivo:  [32P] bio-synthetic labeling, immunoprecipitation, mutation of modification site
 Disease tissue studied:  colorectal cancer, colorectal carcinoma
 Relevant cell lines - cell types - tissues:  293 (epithelial) [KPNA2 (human)], RKO (intestinal)
 Cellular systems studied:  cell lines
 Enzymes shown to modify site in vitro: 
Type Enzyme
KINASE AMPKA1 (human)
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE AMPKA1 (human) transfection of constitutively active enzyme
Downstream Regulation
 Effect of modification (function):  activation, intracellular localization, molecular association, regulation
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
HuR (human) Induces intracellular localization co-immunoprecipitation
 Comments:  promotes the nuclear localization of HuR


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