Curated Information
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Curated Information Page
PubMed Id: 15229287 
Zhang Z, et al. (2004) The phosphorylation of vinculin on tyrosine residues 100 and 1065, mediated by SRC kinases, affects cell spreading. Mol Biol Cell 15, 4234-47 15229287
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
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Y1133-p - Vinculin (human)
Orthologous residues
Vinculin (human): Y1133‑p, Vinculin iso2 (human): Y1065‑p, Vinculin (mouse): Y1065‑p, Vinculin (rat): Y1065‑p, Vinculin (chicken): Y1134‑p, Vinculin (dog):
Characterization
 Methods used to characterize site in vivo phospho-antibody
 Relevant cell lines - cell types - tissues:  platelet-blood
 Cellular systems studied:  primary cells
 Species studied:  human

Y100-p - Vinculin (chicken)
Orthologous residues
Vinculin (human): Y100‑p, Vinculin iso2 (human): Y100‑p, Vinculin (mouse): Y100‑p, Vinculin (rat): Y100‑p, Vinculin (chicken): Y100‑p, Vinculin (dog): Y100‑p
Characterization
 Methods used to characterize site in vivo immunoprecipitation, mutation of modification site
 Relevant cell lines - cell types - tissues:  COS (fibroblast) [Src (human)], COS (fibroblast) [Vinculin (chicken)]
 Cellular systems studied:  cell lines
 Species studied:  monkey
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE Src (human) transfection of constitutively active enzyme
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
vanadate increase
Downstream Regulation
 Effect of modification (function):  protein conformation
 Effect of modification (process):  cell adhesion, altered
 Comments:  phosphorylation of this site positively regulates cell spreading

Y1134-p - Vinculin (chicken)
Orthologous residues
Vinculin (human): Y1133‑p, Vinculin iso2 (human): Y1065‑p, Vinculin (mouse): Y1065‑p, Vinculin (rat): Y1065‑p, Vinculin (chicken): Y1134‑p, Vinculin (dog):
Characterization
 Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, phospho-antibody
 Relevant cell lines - cell types - tissues:  COS (fibroblast) [Src (human)], COS (fibroblast) [Vinculin (chicken)]
 Cellular systems studied:  cell lines
 Species studied:  monkey
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE Src (human)
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE Src (human) transfection of constitutively active enzyme
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
vanadate increase
Downstream Regulation
 Effect of modification (function):  protein conformation
 Effect of modification (process):  cell adhesion, altered
 Comments:  phosphorylation of this site positively regulates cell spreading


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