Curated Information
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Curated Information Page
PubMed Id: 15107605 
King FW, Skeen J, Hay N, Shtivelman E (2004) Inhibition of Chk1 by activated PKB/Akt. Cell Cycle 3, 634-7 15107605
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
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S280-p - Chk1 (human)
Orthologous residues
Chk1 (human): S280‑p, Chk1 (mouse): S280‑p, Chk1 (rat): S280‑p, Chk1 (chicken): S280‑p
Characterization
 Methods used to characterize site in vivo flow cytometry, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  293 (epithelial), HeLa (cervical), MEF (fibroblast) [Akt1 (mouse)], NMB (neuron), SKNSH (neural crest)
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE Akt1 (human) pharmacological inhibitor of upstream enzyme, transfection of wild-type enzyme, phospho-antibody
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
LY294002 decrease
ionizing radiation decrease
hydroxyurea decrease
Downstream Regulation
 Effect of modification (function):  enzymatic activity, inhibited, molecular association, regulation
 Effect of modification (process):  cell cycle regulation


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