|
Orthologous residues
|
|
Chk1 (human): S280‑p, Chk1 (mouse): S280‑p, Chk1 (rat): S280‑p, Chk1 (chicken): S280‑p
|
|
Characterization
|
|
Methods used to characterize site in vivo:
flow cytometry, phospho-antibody, western blotting
|
|
Relevant cell lines - cell types - tissues:
293 (epithelial), HeLa (cervical), MEF (fibroblast) [Akt1 (mouse)], NMB (neuron), SKNSH (neural crest)
|
|
Cellular systems studied:
cell lines
|
|
Species studied:
human
|
|
Upstream Regulation
|
|
Potential in vivo enzymes for site:
|
|
Type
|
Enzyme
|
Evidence
|
Notes
|
|
KINASE
|
Akt1 (human)
|
phospho-antibody, pharmacological inhibitor of upstream enzyme, transfection of wild-type enzyme
|
|
|
|
Treatments, proteins and their effect on site modification:
|
|
Treatments
|
Referenced Treatments
|
Manipulated Protein
|
Referenced Protein
|
Effect
|
Notes
|
|
LY294002
|
|
|
|
decrease
|
|
|
ionizing radiation
|
|
|
|
decrease
|
|
|
hydroxyurea
|
|
|
|
decrease
|
|
|
|
Downstream Regulation
|
|
Effect of modification (function):
enzymatic activity, inhibited, molecular association, regulation
|
|
Effect of modification (process):
cell cycle regulation
|
