Curated Information
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Curated Information Page
PubMed Id: 18703509 
This page summarizes selected information from the article referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Biliran H, et al. (2008) Protein kinase D is a positive regulator of Bit1 apoptotic function. J Biol Chem 283, 28029-37 18703509
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
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S738-p - PKD1 (human)
Orthologous residues
PKD1 (human): S738‑p, PKD1 (mouse): S744‑p, PKD1 (rat): S744‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
fibronectin decrease
polyHEMA increase

S742-p - PKD1 (human)
Orthologous residues
PKD1 (human): S742‑p, PKD1 (mouse): S748‑p, PKD1 (rat): S748‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
fibronectin decrease
polyHEMA increase

S910-p - PKD1 (human)
Orthologous residues
PKD1 (human): S910‑p, PKD1 (mouse): S916‑p, PKD1 (rat): S916‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
fibronectin decrease
polyHEMA increase

S5-p - PTRH2 (human)
Orthologous residues
PTRH2 (human): S5‑p, PTRH2 (mouse): F5‑p, PTRH2 (rat): L5‑p
Characterization
 Methods used to characterize site in vivo immunoprecipitation, mutation of modification site
 Relevant cell lines - cell types - tissues:  293T (epithelial), HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE PKD1 (human) siRNA inhibition of enzyme, transfection of constitutively active enzyme, co-immunoprecipitation, pharmacological activator of upstream enzyme, transfection of inactive enzyme, pharmacological inhibitor of upstream enzyme
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
Go 6976 decrease
Go 6983 no change compared to control
phorbol ester increase
Downstream Regulation
 Effect of modification (function):  intracellular localization
 Effect of modification (process):  apoptosis, altered, cell adhesion, altered
 Comments:  associated with anoikis (cell death induced by detachment from the extracellular matrix)

S87-p - PTRH2 (human)
Orthologous residues
PTRH2 (human): S87‑p, PTRH2 (mouse): S89‑p, PTRH2 (rat): S89‑p
Characterization
 Methods used to characterize site in vivo immunoprecipitation, mutation of modification site
 Relevant cell lines - cell types - tissues:  293T (epithelial), HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  human
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE PKD1 (human)
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE PKD1 (human) siRNA inhibition of enzyme, transfection of constitutively active enzyme, co-immunoprecipitation, pharmacological activator of upstream enzyme, transfection of inactive enzyme, pharmacological inhibitor of upstream enzyme
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
Go 6976 decrease
Go 6983 no change compared to control
phorbol ester increase
Downstream Regulation
 Effect of modification (process):  apoptosis, altered, cell adhesion, altered
 Comments:  associated with anoikis (cell death induced by detachment from the extracellular matrix)


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