Wee HJ, Voon DC, Bae SC, Ito Y (2008) PEBP2-beta/CBF-beta-dependent phosphorylation of RUNX1 and p300 by HIPK2: implications for leukemogenesis. Blood 112, 3777-87 18695000

This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.

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S249-p - AML1 (human) ▲

Modsite: DTRQIQPsPPWsyDQ SwissProt Entrez-Gene Orthologous residues AML1 (human): S249‑p, AML1 iso2 (human): S249‑p, AML1 iso7 (human): S216‑p, AML1 iso8 (human): S276‑p, AML1 iso10 (human): S264‑p, AML1 (mouse): S249‑p, AML1 (rat): S249‑p Characterization Methods used to characterize site in vivo:  mutation of modification site Relevant cell lines - cell types - tissues:  293 (epithelial), COS (fibroblast) Cellular systems studied:  cell lines Species studied:  green monkey, human Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes KINASE HIPK2 (human) transfection of inactive enzyme Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes DYRK1A (human) increase MYH11 (human) decrease Downstream Regulation Effect of modification (function):  molecular association, regulation, phosphorylation Modification regulates interactions with:  Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays CBFB (human) Induces electrophoretic visualization Comments:  promotes AML1 and p300 phosphorylation

S266-p - AML1 (human) ▲

Modsite: QYLGsIAsPsVHPAt SwissProt Entrez-Gene Orthologous residues AML1 (human): S266‑p, AML1 iso2 (human): S266‑p, AML1 iso7 (human): S233‑p, AML1 iso8 (human): S293‑p, AML1 iso10 (human): S281‑p, AML1 (mouse): S266‑p, AML1 (rat): ‑ Characterization Methods used to characterize site in vivo:  mutation of modification site Relevant cell lines - cell types - tissues:  293 (epithelial), COS (fibroblast) Cellular systems studied:  cell lines Species studied:  green monkey, human Downstream Regulation Effect of modification (function):  molecular association, regulation, phosphorylation Modification regulates interactions with:  Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays CBFB (human) Induces electrophoretic visualization Comments:  promotes AML1 and p300 phosphorylation

T273-p - AML1 (human) ▲

Modsite: sPsVHPAtPIsPGRA SwissProt Entrez-Gene Orthologous residues AML1 (human): T273‑p, AML1 iso2 (human): T273‑p, AML1 iso7 (human): T240‑p, AML1 iso8 (human): T300‑p, AML1 iso10 (human): T288‑p, AML1 (mouse): T273‑p, AML1 (rat): T272‑p Characterization Methods used to characterize site in vivo:  mutation of modification site Relevant cell lines - cell types - tissues:  293 (epithelial), COS (fibroblast) Cellular systems studied:  cell lines Species studied:  green monkey, human Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes KINASE HIPK2 (human) transfection of inactive enzyme Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes DYRK1A (human) increase MYH11 (human) decrease Downstream Regulation Effect of modification (function):  molecular association, regulation, phosphorylation Modification regulates interactions with:  Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays CBFB (human) Induces electrophoretic visualization Comments:  promotes AML1 and p300 phosphorylation

S276-p - AML1 (human) ▲

Modsite: VHPAtPIsPGRASGM SwissProt Entrez-Gene Orthologous residues AML1 (human): S276‑p, AML1 iso2 (human): S276‑p, AML1 iso7 (human): ‑, AML1 iso8 (human): S303‑p, AML1 iso10 (human): S291‑p, AML1 (mouse): S276‑p, AML1 (rat): S275‑p Characterization Methods used to characterize site in vivo:  mutation of modification site Relevant cell lines - cell types - tissues:  293 (epithelial), COS (fibroblast) Cellular systems studied:  cell lines Species studied:  green monkey, human Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes KINASE HIPK2 (human) transfection of inactive enzyme Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes DYRK1A (human) increase MYH11 (human) decrease

S831-p - p300 (human) ▲

Modsite: QPALHQNsPsPVPSR SwissProt Entrez-Gene Orthologous residues p300 (human): S831‑p, p300 (mouse): S831‑p, p300 (rat): S831‑p Characterization Methods used to characterize site in vivo:  mutation of modification site Relevant cell lines - cell types - tissues:  293 (epithelial), COS (fibroblast) Cellular systems studied:  cell lines Species studied:  green monkey, human Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes AML1 (human) increase

S833-p - p300 (human) ▲

Modsite: ALHQNsPsPVPSRtP SwissProt Entrez-Gene Orthologous residues p300 (human): S833‑p, p300 (mouse): S833‑p, p300 (rat): S833‑p Characterization Methods used to characterize site in vivo:  mutation of modification site Relevant cell lines - cell types - tissues:  293 (epithelial), COS (fibroblast) Cellular systems studied:  cell lines Species studied:  green monkey, human Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes AML1 (human) increase

T839-p - p300 (human) ▲

Modsite: PsPVPSRtPtPHHtP SwissProt Entrez-Gene Orthologous residues p300 (human): T839‑p, p300 (mouse): T839‑p, p300 (rat): T839‑p Characterization Methods used to characterize site in vivo:  mutation of modification site Relevant cell lines - cell types - tissues:  293 (epithelial), COS (fibroblast) Cellular systems studied:  cell lines Species studied:  green monkey, human Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes AML1 (human) increase

T841-p - p300 (human) ▲

Modsite: PVPSRtPtPHHtPPS SwissProt Entrez-Gene Orthologous residues p300 (human): T841‑p, p300 (mouse): T841‑p, p300 (rat): T841‑p Characterization Methods used to characterize site in vivo:  mutation of modification site Relevant cell lines - cell types - tissues:  293 (epithelial), COS (fibroblast) Cellular systems studied:  cell lines Species studied:  green monkey, human Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes AML1 (human) increase

T845-p - p300 (human) ▲

Modsite: RtPtPHHtPPSIGAQ SwissProt Entrez-Gene Orthologous residues p300 (human): T845‑p, p300 (mouse): T845‑p, p300 (rat): T845‑p Characterization Methods used to characterize site in vivo:  mutation of modification site Relevant cell lines - cell types - tissues:  293 (epithelial), COS (fibroblast) Cellular systems studied:  cell lines Species studied:  green monkey, human Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes AML1 (human) increase

T885-p - p300 (human) ▲

Modsite: LHPPPRQtPtPPTtQ SwissProt Entrez-Gene Orthologous residues p300 (human): T885‑p, p300 (mouse): T885‑p, p300 (rat): T885‑p Characterization Methods used to characterize site in vivo:  mutation of modification site Relevant cell lines - cell types - tissues:  293 (epithelial), COS (fibroblast) Cellular systems studied:  cell lines Species studied:  green monkey, human Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes AML1 (human) increase

T887-p - p300 (human) ▲

Modsite: PPPRQtPtPPTtQLP SwissProt Entrez-Gene Orthologous residues p300 (human): T887‑p, p300 (mouse): T887‑p, p300 (rat): T887‑p Characterization Methods used to characterize site in vivo:  mutation of modification site Relevant cell lines - cell types - tissues:  293 (epithelial), COS (fibroblast) Cellular systems studied:  cell lines Species studied:  green monkey, human Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes AML1 (human) increase