|
Orthologous residues
|
|
TIE2 (human): Y1102‑p, TIE2 (mouse): Y1100‑p
|
|
Characterization
|
|
Methods used to characterize site in vivo:
[32P] bio-synthetic labeling, immunoprecipitation, mutation of modification site, phospho-antibody
|
|
Disease tissue studied:
type 2 diabetes
|
|
Relevant cell lines - cell types - tissues:
293T (epithelial) [TIE2 (mouse)]
|
|
Cellular systems studied:
cell lines
|
|
Species studied:
human
|
|
Upstream Regulation
|
|
Potential in vivo enzymes for site:
|
|
Type
|
Enzyme
|
Evidence
|
Notes
|
|
KINASE
|
TIE2 (mouse)
|
transfection of inactive enzyme
|
|
|
|
Downstream Regulation
|
|
Effect of modification (function):
molecular association, regulation
|
|
Modification regulates interactions with:
|
|
Interacting molecule
|
Interacting domains
|
Effect
|
Consequences (function)
|
Consequences (process)
|
Detection assays
|
|
PIK3R1 (mouse)
|
|
Induces
|
activation
|
|
yeast two-hybrid, co-immunoprecipitation
|
|
GRB14 (mouse)
|
|
Induces
|
activation
|
|
yeast two-hybrid
|
|
Grb2 (mouse)
|
|
Induces
|
activation
|
|
yeast two-hybrid, co-immunoprecipitation
|
|
SHP-2 (mouse)
|
|
Induces
|
activation
|
|
yeast two-hybrid
|
|
Grb7 (mouse)
|
|
Induces
|
activation
|
|
yeast two-hybrid, co-immunoprecipitation
|
|
