Curated Information
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Curated Information Page
PubMed Id: 15170389 
This page summarizes selected information from the article referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Arnaud M, et al. (2004) Interaction of the tyrosine phosphatase SHP-2 with Gab2 regulates Rho-dependent activation of the c-fos serum response element by interleukin-2. Biochem J 382, 545-56 15170389
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
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Y614-p - Gab2 (human)
Orthologous residues
Gab2 (human): Y614‑p, Gab2 (mouse): Y603‑p, Gab2 (rat): Y603‑p
Characterization
 Methods used to characterize site in vivo mutation of modification site, phospho-antibody, western blotting
 Disease tissue studied:  leukemia, T cell leukemia
 Relevant cell lines - cell types - tissues:  Kit225 (T lymphocyte)
 Cellular systems studied:  cell lines
 Species studied:  human
 Comments:  Phosphoantibody is anti-pTyr + point mutations.
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
IL-2 increase
Downstream Regulation
 Effect of modification (function):  molecular association, regulation
 Effect of modification (process):  cell cycle regulation, transcription, altered
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
SHP-2 (human) SH2 Induces transcription, altered, cell cycle regulation pull-down assay, co-immunoprecipitation, far-Western
 Comments:  Binding of SHP-2 activates the MAPK pathway and transcription.

Y643-p - Gab2 (human)
Orthologous residues
Gab2 (human): Y643‑p, Gab2 (mouse): Y632‑p, Gab2 (rat): Y632‑p
Characterization
 Methods used to characterize site in vivo mutation of modification site, phospho-antibody, western blotting
 Disease tissue studied:  leukemia, T cell leukemia
 Relevant cell lines - cell types - tissues:  Kit225 (T lymphocyte)
 Cellular systems studied:  cell lines
 Species studied:  human
 Comments:  Phosphoantibody is anti-pTyr + point mutations.
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
IL-2 increase
Downstream Regulation
 Effect of modification (function):  molecular association, regulation
 Effect of modification (process):  cell cycle regulation, transcription, altered
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
SHP-2 (human) SH2 Induces transcription, altered, cell cycle regulation pull-down assay, co-immunoprecipitation, far-Western
 Comments:  Binding of SHP-2 activates the MAPK pathway and transcription.


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