Curated Information
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Curated Information Page
PubMed Id: 15100278 
This page summarizes selected information from the article referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Goda S, et al. (2004) Control of TCR-mediated activation of beta 1 integrins by the ZAP-70 tyrosine kinase interdomain B region and the linker for activation of T cells adapter protein. J Immunol 172, 5379-87 15100278
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
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Y171-p - LAT iso2 (human) ▲
Orthologous residues
LAT (human): Y200‑p, LAT iso2 (human): Y171‑p, LAT (mouse): Y175‑p, LAT (rat): Y175‑p
Characterization
 Methods used to characterize site in vivo:  mutation of modification site, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  Jurkat (T lymphocyte), P116 (T lymphocyte)
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
anti-CD3 increase
ZAP70 (human) increase
Downstream Regulation
 Effect of modification (function):  activation
 Effect of modification (process):  cell adhesion, altered

Y191-p - LAT iso2 (human) ▲
Orthologous residues
LAT (human): Y220‑p, LAT iso2 (human): Y191‑p, LAT (mouse): Y195‑p, LAT (rat): Y195‑p
Characterization
 Methods used to characterize site in vivo:  mutation of modification site, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  Jurkat (T lymphocyte), P116 (T lymphocyte)
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
anti-CD3 increase
ZAP70 (human) increase
Downstream Regulation
 Effect of modification (function):  activation
 Effect of modification (process):  cell adhesion, altered

Y292-p - ZAP70 (human) ▲
Orthologous residues
ZAP70 (human): Y292‑p, ZAP70 (mouse): Y290‑p, ZAP70 (rat): Y290‑p
Characterization
 Methods used to characterize site in vivo:  mutation of modification site, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  Jurkat (T lymphocyte), P116 (T lymphocyte)
 Cellular systems studied:  cell lines
 Species studied:  human
Downstream Regulation
 Effect of modification (function):  enzymatic activity, inhibited, phosphorylation
 Effect of modification (process):  cell adhesion, altered
 Comments:  inhibits PI3K activation and adhesion; regulates phosphorylation of LAT Y171 and Y191

Y315-p - ZAP70 (human) ▲
Orthologous residues
ZAP70 (human): Y315‑p, ZAP70 (mouse): Y314‑p, ZAP70 (rat): Y310‑p
Characterization
 Methods used to characterize site in vivo:  mutation of modification site, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  Jurkat (T lymphocyte), P116 (T lymphocyte)
 Cellular systems studied:  cell lines
 Species studied:  human
Downstream Regulation
 Effect of modification (function):  enzymatic activity, induced, phosphorylation
 Effect of modification (process):  cell adhesion, altered
 Comments:  increases PI3K activation and adhesion; regulates phosphorylation of LAT Y171 and Y191

Y493-p - ZAP70 (human) ▲
Orthologous residues
ZAP70 (human): Y493‑p, ZAP70 (mouse): Y492‑p, ZAP70 (rat): Y488‑p
Characterization
 Methods used to characterize site in vivo:  mutation of modification site, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  Jurkat (T lymphocyte), P116 (T lymphocyte)
 Cellular systems studied:  cell lines
 Species studied:  human
Downstream Regulation
 Effect of modification (function):  enzymatic activity, induced, phosphorylation
 Effect of modification (process):  cell adhesion, altered
 Comments:  increases PI3K activation and adhesion; regulates phosphorylation of LAT Y171 and Y191


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