Curated Information
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Curated Information Page
PubMed Id: 15143187 
This page summarizes selected information from the article referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Argetsinger LS, et al. (2004) Autophosphorylation of JAK2 on tyrosines 221 and 570 regulates its activity. Mol Cell Biol 24, 4955-67 15143187
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
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Y221-p - JAK2 (mouse)
Orthologous residues
JAK2 (human): Y221‑p, JAK2 (mouse): Y221‑p, JAK2 (rat): Y221‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry, mutation of modification site, phosphopeptide mapping
 Relevant cell lines - cell types - tissues:  3T3 (fibroblast) [JAK2 (mouse)]
 Cellular systems studied:  cell lines
 Species studied:  mouse
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE JAK2 (mouse)
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE JAK2 (mouse) 2D analysis, phosphopeptide analysis
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
GH increase
Downstream Regulation
 Effect of modification (function):  enzymatic activity, induced

Y570-p - JAK2 (mouse)
Orthologous residues
JAK2 (human): Y570‑p, JAK2 (mouse): Y570‑p, JAK2 (rat): Y570‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry, mutation of modification site, phosphopeptide mapping
 Relevant cell lines - cell types - tissues:  3T3 (fibroblast) [JAK2 (mouse)]
 Cellular systems studied:  cell lines
 Species studied:  mouse
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE JAK2 (mouse)
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE JAK2 (mouse) 2D analysis, phosphopeptide analysis
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
GH increase
Downstream Regulation
 Effect of modification (function):  enzymatic activity, inhibited

Y1007-p - JAK2 (mouse)
Orthologous residues
JAK2 (human): Y1007‑p, JAK2 (mouse): Y1007‑p, JAK2 (rat): Y1007‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry, phospho-antibody, phosphopeptide mapping
 Relevant cell lines - cell types - tissues:  3T3 (fibroblast) [JAK2 (mouse)]
 Cellular systems studied:  cell lines
 Species studied:  mouse
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE JAK2 (mouse)

Y1008-p - JAK2 (mouse)
Orthologous residues
JAK2 (human): Y1008‑p, JAK2 (mouse): Y1008‑p, JAK2 (rat): Y1008‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human

Y699-p - STAT5B (rat)
Orthologous residues
STAT5B (human): Y699‑p, STAT5B (mouse): Y699‑p, STAT5B (rat): Y699‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE JAK2 (mouse) transfection of wild-type enzyme, transfection of inactive enzyme


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