Curated Information
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PubMed Id: 15121872 
Kurzer JH, et al. (2004) Tyrosine 813 is a site of JAK2 autophosphorylation critical for activation of JAK2 by SH2-B beta. Mol Cell Biol 24, 4557-70 15121872
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
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Y785-p - JAK3 (human)
Orthologous residues
JAK3 (human): Y785‑p, JAK3 (mouse): Y781‑p, JAK3 iso2 (mouse): Y1000‑p, JAK3 iso3 (mouse): Y996‑p, JAK3 (rat): Y781‑p
Characterization
 Methods used to characterize site in vivo mutation of modification site, phospho-antibody
 Relevant cell lines - cell types - tissues:  COS (fibroblast), NK 3.3 (natural killer cell)
 Cellular systems studied:  cell lines
 Species studied:  human, monkey
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE JAK3 (human)
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
IL-2 increase
Downstream Regulation
 Effect of modification (function):  molecular association, regulation
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
SH2-B-beta (rat) Induces co-immunoprecipitation

Y813-p - JAK2 (mouse)
Orthologous residues
JAK2 (human): Y813‑p, JAK2 (mouse): Y813‑p, JAK2 (rat): Y813‑p
Characterization
 Methods used to characterize site in vivo [32P] bio-synthetic labeling, mutation of modification site, phospho-antibody, phosphoamino acid analysis, phosphopeptide mapping
 Relevant cell lines - cell types - tissues:  293T (epithelial), 3T3 (fibroblast) [SHP-2 (mouse), homozygous knockout], COS (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  human, monkey, mouse
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE JAK2 (mouse)
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
GH increase
Downstream Regulation
 Effect of modification (function):  molecular association, regulation
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
SH2-B-beta (rat) Induces co-immunoprecipitation

Y699-p - STAT5B (rat)
Orthologous residues
STAT5B (human): Y699‑p, STAT5B (mouse): Y699‑p, STAT5B (rat): Y699‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody
 Relevant cell lines - cell types - tissues:  COS (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  monkey
 Comments:  Co-expression with Jak2 and SH2-Bbeta enhances phosphorylation of STAT5B.


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