Curated Information
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Curated Information Page
PubMed Id: 15109498 
This page summarizes selected information from the article referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Yang X, et al. (2004) ATF4 is a substrate of RSK2 and an essential regulator of osteoblast biology; implication for Coffin-Lowry Syndrome. Cell 117, 387-98 15109498
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
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S245-p - ATF-4 (human)
Orthologous residues
ATF‑4 (human): S245‑p, ATF‑4 (mouse): N244‑p, ATF‑4 (rat): S243‑p
Characterization
 Methods used to characterize site in vivo [32P] bio-synthetic labeling, electrophoretic mobility shift, immunoprecipitation, phospho-antibody
 Relevant cell lines - cell types - tissues:  osteoblast-calvarium
 Cellular systems studied:  primary cells
 Species studied:  mouse
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE RSK2 (human)
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE RSK2 (human) transfection of wild-type enzyme, genetic knockout/knockin of upstream enzyme
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
phorbol ester increase
Downstream Regulation
 Effect of modification (function):  activity, induced
 Effect of modification (process):  cell growth, altered

S251-p - ATF-4 (mouse)
Orthologous residues
ATF‑4 (human): L252‑p, ATF‑4 (mouse): S251‑p, ATF‑4 (rat): P250‑p
Characterization
 Methods used to characterize site in vivo [32P] bio-synthetic labeling, electrophoretic mobility shift, immunoprecipitation, phospho-antibody
 Relevant cell lines - cell types - tissues:  osteoblast-calvarium
 Cellular systems studied:  primary cells
 Species studied:  mouse
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE RSK2 (mouse)
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE RSK2 (mouse) transfection of wild-type enzyme, genetic knockout/knockin of upstream enzyme
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
phorbol ester increase
Downstream Regulation
 Effect of modification (function):  activity, induced
 Effect of modification (process):  cell growth, altered


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