Curated Information
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PubMed Id: 14996911 
Mariner DJ, Davis MA, Reynolds AB (2004) EGFR signaling to p120-catenin through phosphorylation at Y228. J Cell Sci 117, 1339-50 14996911
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
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Y228-p - CTNND1 (mouse)
Orthologous residues
CTNND1 (human): Y228‑p, CTNND1 iso2 (human): Y228‑p, CTNND1 iso3 (human): Y228‑p, CTNND1 iso4 (human): Y228‑p, CTNND1 iso10 (human): Y174‑p, CTNND1 iso22 (human): Y127‑p, CTNND1 (mouse): Y228‑p, CTNND1 iso3 (mouse): Y228‑p, CTNND1 (rat): Y228‑p
 Methods used to characterize site in vivo electrophoretic mobility shift, mutation of modification site, phospho-antibody, western blotting
 Disease tissue studied:  breast cancer, colorectal cancer, colorectal carcinoma, pancreatic cancer, pancreatic carcinoma
 Relevant cell lines - cell types - tissues:  3T3 (fibroblast), A431 (epithelial), HCT116 (intestinal), HT-29 (intestinal), MDA-MB453 (breast cell), MDA-MB468 (breast cell), MIA PaCa-2 (pancreatic), SKBr3 (breast cell), SYF (fibroblast), T47D (breast cell)
 Cellular systems studied:  cell lines
 Species studied:  human, mouse
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE Src (human)
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE Src (human) pharmacological inhibitor of upstream enzyme, transfection of inactive enzyme, phospho-antibody, transfection of wild-type enzyme, transfection of constitutively active enzyme, pharmacological activator of upstream enzyme
 Comments:  not the only kinase as SYF cells still exhibit robust pY228 in +EGF
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
EGF increase
SU6656 EGF inhibit treatment-induced increase slight decrease
SU6656 decrease
AG1478 decrease
EKI-785 decrease
PP1 decrease
low Ca(2+) decrease

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