|
Orthologous residues
|
|
IRS1 (human): S323‑p, IRS1 (mouse): S318‑p, IRS1 (rat): S318‑p
|
|
Characterization
|
|
Methods used to characterize site in vivo:
mutation of modification site, phospho-antibody
|
|
Relevant cell lines - cell types - tissues:
BHK-21 (fibroblast)
|
|
Cellular systems studied:
cell lines
|
|
Species studied:
hamster
|
|
Enzymes shown to modify site in vitro:
|
|
|
|
Upstream Regulation
|
|
Potential in vivo enzymes for site:
|
|
Type
|
Enzyme
|
Evidence
|
Notes
|
|
KINASE
|
PKCZ (rat)
|
transfection of wild-type enzyme, phospho-antibody, activation of upstream enzyme, mutation in upstream enzyme recognition motif
|
|
|
|
Treatments, proteins and their effect on site modification:
|
|
Treatments
|
Referenced Treatments
|
Manipulated Protein
|
Referenced Protein
|
Effect
|
Notes
|
|
insulin
|
|
|
|
increase
|
|
|
|
Downstream Regulation
|
|
Effect of modification (function):
molecular association, regulation, phosphorylation
|
|
Modification regulates interactions with:
|
|
Interacting molecule
|
Interacting domains
|
Effect
|
Consequences (function)
|
Consequences (process)
|
Detection assays
|
|
InsR (rat)
|
|
Disrupts
|
|
|
|
|
|
Comments:
Inhibits tyrosine phosphorylation
|
