Curated Information
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PubMed Id: 15024069 
This page summarizes selected information from the article referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Gómez-del Arco P, Maki K, Georgopoulos K (2004) Phosphorylation controls Ikaros's ability to negatively regulate the G(1)-S transition. Mol Cell Biol 24, 2797-807 15024069
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
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S63-p - Ikaros (mouse) ▲
Orthologous residues
Ikaros (human): S63‑p, Ikaros iso12 (human): ‑, Ikaros (mouse): S63‑p, Ikaros iso9 (mouse): S83‑p
Characterization
 Methods used to characterize site in vivo:  [32P] bio-synthetic labeling, electrophoretic mobility shift, mutation of modification site, phosphopeptide mapping
 Disease tissue studied:  lymphoma, B cell lymphoma
 Relevant cell lines - cell types - tissues:  293T (epithelial), 3T3 (fibroblast) [SHP-2 (mouse), homozygous knockout], Bal 17 (B lymphocyte)
 Cellular systems studied:  cell lines
 Species studied:  human, mouse
 Comments:  Rml11; T-cell line 510
 Enzymes shown to modify site in vitro: 
Type Enzyme
KINASE CK2A1 (human)
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
mimosine decrease
Downstream Regulation
 Effect of modification (function):  inhibition
 Effect of modification (process):  cell cycle regulation
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
DNA Disrupts

S384-p - Ikaros (mouse) ▲
Orthologous residues
Ikaros (human): S389‑p, Ikaros iso12 (human): S164‑p, Ikaros (mouse): S384‑p, Ikaros iso9 (mouse): S405‑p
Characterization
 Methods used to characterize site in vivo:  [32P] bio-synthetic labeling, electrophoretic mobility shift, mutation of modification site, phosphopeptide mapping
 Disease tissue studied:  lymphoma, B cell lymphoma
 Relevant cell lines - cell types - tissues:  293T (epithelial), 3T3 (fibroblast) [SHP-2 (mouse), homozygous knockout], Bal 17 (B lymphocyte)
 Cellular systems studied:  cell lines
 Species studied:  human, mouse
 Comments:  Rml11; T-cell line 510
Downstream Regulation
 Effect of modification (function):  inhibition
 Effect of modification (process):  cell cycle regulation
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
DNA Disrupts

S386-p - Ikaros (mouse) ▲
Orthologous residues
Ikaros (human): S391‑p, Ikaros iso12 (human): S166‑p, Ikaros (mouse): S386‑p, Ikaros iso9 (mouse): S407‑p
Characterization
 Methods used to characterize site in vivo:  [32P] bio-synthetic labeling, electrophoretic mobility shift, mutation of modification site, phosphopeptide mapping
 Disease tissue studied:  lymphoma, B cell lymphoma
 Relevant cell lines - cell types - tissues:  293T (epithelial), 3T3 (fibroblast) [SHP-2 (mouse), homozygous knockout], Bal 17 (B lymphocyte)
 Cellular systems studied:  cell lines
 Species studied:  human, mouse
 Comments:  Rml11; T-cell line 510
Downstream Regulation
 Effect of modification (function):  inhibition
 Effect of modification (process):  cell cycle regulation
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
DNA Disrupts

S388-p - Ikaros (mouse) ▲
Orthologous residues
Ikaros (human): S393‑p, Ikaros iso12 (human): S168‑p, Ikaros (mouse): S388‑p, Ikaros iso9 (mouse): S409‑p
Characterization
 Methods used to characterize site in vivo:  [32P] bio-synthetic labeling, electrophoretic mobility shift, mutation of modification site, phosphopeptide mapping
 Disease tissue studied:  lymphoma, B cell lymphoma
 Relevant cell lines - cell types - tissues:  293T (epithelial), 3T3 (fibroblast) [SHP-2 (mouse), homozygous knockout], Bal 17 (B lymphocyte)
 Cellular systems studied:  cell lines
 Species studied:  human, mouse
 Comments:  Rml11; T-cell line 510
 Enzymes shown to modify site in vitro: 
Type Enzyme
KINASE CK2A1 (human)
Downstream Regulation
 Effect of modification (function):  inhibition
 Effect of modification (process):  cell cycle regulation
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
DNA Disrupts

S392-p - Ikaros (mouse) ▲
Orthologous residues
Ikaros (human): S397‑p, Ikaros iso12 (human): S172‑p, Ikaros (mouse): S392‑p, Ikaros iso9 (mouse): S413‑p
Characterization
 Methods used to characterize site in vivo:  [32P] bio-synthetic labeling, electrophoretic mobility shift, mutation of modification site, phosphopeptide mapping
 Disease tissue studied:  lymphoma, B cell lymphoma
 Relevant cell lines - cell types - tissues:  293T (epithelial), 3T3 (fibroblast) [SHP-2 (mouse), homozygous knockout], Bal 17 (B lymphocyte)
 Cellular systems studied:  cell lines
 Species studied:  human, mouse
 Comments:  Rml11; T-cell line 510
Downstream Regulation
 Effect of modification (function):  inhibition
 Effect of modification (process):  cell cycle regulation
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
DNA Disrupts

T393-p - Ikaros (mouse) ▲
Orthologous residues
Ikaros (human): T398‑p, Ikaros iso12 (human): T173‑p, Ikaros (mouse): T393‑p, Ikaros iso9 (mouse): T414‑p
Characterization
 Methods used to characterize site in vivo:  [32P] bio-synthetic labeling, electrophoretic mobility shift, mutation of modification site, phosphopeptide mapping
 Disease tissue studied:  lymphoma, B cell lymphoma
 Relevant cell lines - cell types - tissues:  293T (epithelial), 3T3 (fibroblast) [SHP-2 (mouse), homozygous knockout], Bal 17 (B lymphocyte)
 Cellular systems studied:  cell lines
 Species studied:  human, mouse
 Comments:  Rml11; T-cell line 510
 Enzymes shown to modify site in vitro: 
Type Enzyme
KINASE CK2A1 (human)
Downstream Regulation
 Effect of modification (function):  inhibition
 Effect of modification (process):  cell cycle regulation
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
DNA Disrupts


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