Curated Information
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Curated Information Page
PubMed Id: 11909964 
Shiraha H, et al. (2002) Activation of m-calpain (calpain II) by epidermal growth factor is limited by protein kinase A phosphorylation of m-calpain. Mol Cell Biol 22, 2716-27 11909964
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
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S369-p - Calpain-2 (human)
Orthologous residues
Calpain‑2 (human): S369‑p, Calpain‑2 (mouse): S369‑p, Calpain‑2 (rat): S369‑p
Characterization
 Methods used to characterize site in vivo [32P] bio-synthetic labeling, mutation of modification site
 Relevant cell lines - cell types - tissues:  NR6WT (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  mouse
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE PKACA (human) pharmacological inhibitor of upstream enzyme
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
cAMP analog increase
H-89 cAMP analog inhibit treatment-induced increase
8-Rp-cAMP cAMP analog inhibit treatment-induced increase
Downstream Regulation
 Effect of modification (function):  activity, inhibited
 Effect of modification (process):  cell motility, altered

T370-p - Calpain-2 (human)
Orthologous residues
Calpain‑2 (human): T370‑p, Calpain‑2 (mouse): T370‑p, Calpain‑2 (rat): T370‑p
Characterization
 Methods used to characterize site in vivo [32P] bio-synthetic labeling, mutation of modification site
 Relevant cell lines - cell types - tissues:  NR6WT (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  mouse
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE PKACA (human)
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE PKACA (human) pharmacological inhibitor of upstream enzyme
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
cAMP analog increase
H-89 cAMP analog inhibit treatment-induced increase
8-Rp-cAMP cAMP analog inhibit treatment-induced increase
Downstream Regulation
 Effect of modification (function):  activity, inhibited
 Effect of modification (process):  cell motility, altered


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