|
Orthologous residues
|
|
eIF4B (human): S422‑p, eIF4B (mouse): S422‑p, eIF4B (rat): S422‑p
|
|
Characterization
|
|
Methods used to characterize site in vivo:
[32P] bio-synthetic labeling, mutation of modification site, phospho-antibody, phosphoamino acid analysis, phosphopeptide mapping, western blotting
|
|
Relevant cell lines - cell types - tissues:
293 (epithelial), 293T (epithelial), HeLa (cervical)
|
|
Cellular systems studied:
cell lines
|
|
Species studied:
human
|
|
Upstream Regulation
|
|
Potential in vivo enzymes for site:
|
|
Type
|
Enzyme
|
Evidence
|
Notes
|
|
KINASE
|
p70S6K (human)
|
transfection of constitutively active enzyme, transfection of wild-type enzyme
|
|
|
|
Treatments, proteins and their effect on site modification:
|
|
Treatments
|
Referenced Treatments
|
Manipulated Protein
|
Referenced Protein
|
Effect
|
Notes
|
|
serum
|
|
|
|
increase
|
|
|
rapamycin
|
serum
|
|
|
inhibit treatment-induced increase
|
|
|
LY294002
|
serum
|
|
|
inhibit treatment-induced increase
|
|
|
wortmannin
|
serum
|
|
|
inhibit treatment-induced increase
|
|
|
amino acid starvation
|
|
|
|
decrease
|
|
|
|
Downstream Regulation
|
|
Effect of modification (function):
inhibition
|
|
Effect of modification (process):
translation, altered
|
